Semisynthetic sensor proteins enable metabolic assays at the point of care.

Détails

ID Serval
serval:BIB_399ED9288473
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Semisynthetic sensor proteins enable metabolic assays at the point of care.
Périodique
Science
Auteur⸱e⸱s
Yu Q., Xue L., Hiblot J., Griss R., Fabritz S., Roux C., Binz P.A., Haas D., Okun J.G., Johnsson K.
ISSN
1095-9203 (Electronic)
ISSN-L
0036-8075
Statut éditorial
Publié
Date de publication
14/09/2018
Peer-reviewed
Oui
Volume
361
Numéro
6407
Pages
1122-1126
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
Monitoring metabolites at the point of care could improve the diagnosis and management of numerous diseases. Yet for most metabolites, such assays are not available. We introduce semisynthetic, light-emitting sensor proteins for use in paper-based metabolic assays. The metabolite is oxidized by nicotinamide adenine dinucleotide phosphate, and the sensor changes color in the presence of the reduced cofactor, enabling metabolite quantification with the use of a digital camera. The approach makes any metabolite that can be oxidized by the cofactor a candidate for quantitative point-of-care assays, as shown for phenylalanine, glucose, and glutamate. Phenylalanine blood levels of phenylketonuria patients were analyzed at the point of care within minutes with only 0.5 microliters of blood. Results were within 15% of those obtained with standard testing methods.
Mots-clé
Bioluminescence Resonance Energy Transfer Techniques, Biosensing Techniques, Blood Glucose/analysis, Escherichia coli Proteins/chemistry, Escherichia coli Proteins/genetics, Glutamic Acid/blood, Humans, Monitoring, Physiologic/methods, NADP/metabolism, Oxidation-Reduction, Phenylalanine/blood, Phenylketonurias/blood, Phenylketonurias/diagnosis, Point-of-Care Testing, Tetrahydrofolate Dehydrogenase/chemistry, Tetrahydrofolate Dehydrogenase/genetics
Pubmed
Web of science
Création de la notice
05/10/2018 17:33
Dernière modification de la notice
20/08/2019 14:29
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