Use of the pyrophosphatase activity as a reliable tonoplast marker in maize roots.

Détails

ID Serval
serval:BIB_3871
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Use of the pyrophosphatase activity as a reliable tonoplast marker in maize roots.
Périodique
Plant Science
Auteur(s)
Chanson A.
ISSN
0168-9452
Statut éditorial
Publié
Date de publication
1990
Volume
71
Numéro
2
Pages
199-207
Langue
anglais
Résumé
Membranes of maize (Zea mays L., cv LG 11) roots were fractionated by sucrose (in presence or absence of Mg2+) or dextran density gradient centrifugations and the locations of organelles were determined using marker enzymes. Latent UDPase was used as a Golgi marker, catalase for the peroxysomes, cytochrome c oxidase for the mitochondria, UDP-Gal-galactosyltransferase for the amyloplast membranes and NADH-cytochrome c reductase for the ER. Two markers were selected for the plasmalemma, the vanadate-sensitive ATPase and UDP-Glc-sterolglucosyltransferase. The distributions of the PPase and vacuolar ATPase were found to be similar after density gradient centrifugation. The PPase and vacuolar ATPase activities were clearly separated from almost all the other markers tested, however, a partial association of both activities with the ER cannot be completely ruled out. The PPase of maize roots is more active and easier to measure than the vacuolar ATPase and is therefore an excellent candidate for use as a tonoplast marker.
Mots-clé
ATPase, pyrophosphatase, marker enzymes, tonoplast, root, Zea mays L.
Web of science
Création de la notice
19/11/2007 11:14
Dernière modification de la notice
20/08/2019 14:27
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