Human protection of telomeres 1 (POT1) is a negative regulator of telomerase activity in vitro.

Détails

ID Serval
serval:BIB_37919
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Human protection of telomeres 1 (POT1) is a negative regulator of telomerase activity in vitro.
Périodique
Molecular and Cellular Biology
Auteur(s)
Kelleher C., Kurth I., Lingner J.
ISSN
0270-7306 (Print)
ISSN-L
0270-7306
Statut éditorial
Publié
Date de publication
2005
Volume
25
Numéro
2
Pages
808-818
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
The telomeric single-strand DNA binding protein protection of telomeres 1 (POT1) protects telomeres from rapid degradation in Schizosaccharomyces pombe and has been implicated in positive and negative telomere length regulation in humans. Human POT1 appears to interact with telomeres both through direct binding to the 3' overhanging G-strand DNA and through interaction with the TRF1 duplex telomere DNA binding complex. The influence of POT1 on telomerase activity has not been studied at the molecular level. We show here that POT1 negatively effects telomerase activity in vitro. We find that the DNA binding activity of POT1 is required for telomerase inhibition. Furthermore, POT1 is incapable of inhibiting telomeric repeat addition to substrate primers that are defective for POT1 binding, suggesting that in vivo, POT1 likely affects substrate access to telomerase.
Mots-clé
Animals, Binding Sites, DNA, Single-Stranded/metabolism, DNA-Binding Proteins/genetics, DNA-Binding Proteins/metabolism, HeLa Cells, Humans, Protein Binding, Protein Structure, Tertiary, Telomerase/antagonists & inhibitors, Telomerase/genetics, Telomere/metabolism, Telomere-Binding Proteins/genetics, Telomere-Binding Proteins/metabolism
Pubmed
Web of science
Open Access
Oui
Création de la notice
19/11/2007 13:36
Dernière modification de la notice
20/08/2019 14:26
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