Human protection of telomeres 1 (POT1) is a negative regulator of telomerase activity in vitro.

Details

Serval ID
serval:BIB_37919
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Human protection of telomeres 1 (POT1) is a negative regulator of telomerase activity in vitro.
Journal
Molecular and Cellular Biology
Author(s)
Kelleher C., Kurth I., Lingner J.
ISSN
0270-7306 (Print)
ISSN-L
0270-7306
Publication state
Published
Issued date
2005
Volume
25
Number
2
Pages
808-818
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
The telomeric single-strand DNA binding protein protection of telomeres 1 (POT1) protects telomeres from rapid degradation in Schizosaccharomyces pombe and has been implicated in positive and negative telomere length regulation in humans. Human POT1 appears to interact with telomeres both through direct binding to the 3' overhanging G-strand DNA and through interaction with the TRF1 duplex telomere DNA binding complex. The influence of POT1 on telomerase activity has not been studied at the molecular level. We show here that POT1 negatively effects telomerase activity in vitro. We find that the DNA binding activity of POT1 is required for telomerase inhibition. Furthermore, POT1 is incapable of inhibiting telomeric repeat addition to substrate primers that are defective for POT1 binding, suggesting that in vivo, POT1 likely affects substrate access to telomerase.
Keywords
Animals, Binding Sites, DNA, Single-Stranded/metabolism, DNA-Binding Proteins/genetics, DNA-Binding Proteins/metabolism, HeLa Cells, Humans, Protein Binding, Protein Structure, Tertiary, Telomerase/antagonists & inhibitors, Telomerase/genetics, Telomere/metabolism, Telomere-Binding Proteins/genetics, Telomere-Binding Proteins/metabolism
Pubmed
Web of science
Open Access
Yes
Create date
19/11/2007 12:36
Last modification date
20/08/2019 13:26
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