Analysis, Isolation, and Activation of Antigen-Specific CD4 + and CD8+ T Cells by Soluble MHC-Peptide Complexes

Détails

Ressource 1Télécharger: BIB_36B69997AF74.P001.pdf (1591.54 [Ko])
Etat: Public
Version: Final published version
ID Serval
serval:BIB_36B69997AF74
Type
Article: article d'un périodique ou d'un magazine.
Sous-type
Synthèse (review): revue aussi complète que possible des connaissances sur un sujet, rédigée à partir de l'analyse exhaustive des travaux publiés.
Collection
Publications
Institution
Titre
Analysis, Isolation, and Activation of Antigen-Specific CD4 + and CD8+ T Cells by Soluble MHC-Peptide Complexes
Périodique
Frontiers In Immunology
Auteur⸱e⸱s
Schmidt J., Dojcinovic D., Guillaume P., Luescher I.
ISSN
1664-3224 (Electronic)
ISSN-L
1664-3224
Statut éditorial
Publié
Date de publication
2013
Peer-reviewed
Oui
Volume
4
Pages
218
Langue
anglais
Notes
Publication types: Immunology ; review-article Identifiant PubMed Central: PMC3726995
Résumé
T cells constitute the core of adaptive cellular immunity and protect higher organisms against pathogen infections and cancer. Monitoring of disease progression as well as prophylactic or therapeutic vaccines and immunotherapies call for conclusive detection, analysis, and sorting of antigen-specific T cells. This is possible by means of soluble recombinant ligands for T cells, i.e., MHC class I-peptide (pMHC I) complexes for CD8(+) T cells and MHC class II-peptide (pMHC II) complexes for CD4(+) T cells and flow cytometry. Here we review major developments in the development of pMHC staining reagents and their diverse applications and discuss perspectives of their use for basic and clinical investigations.
Mots-clé
T cells, T cell receptor, tetramers, MHC, flow cytometry, coreceptor
Pubmed
Web of science
Open Access
Oui
Création de la notice
11/07/2016 10:05
Dernière modification de la notice
20/08/2019 13:24
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