Analysis, Isolation, and Activation of Antigen-Specific CD4 + and CD8+ T Cells by Soluble MHC-Peptide Complexes

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Ressource 1Download: BIB_36B69997AF74.P001.pdf (1591.54 [Ko])
State: Public
Version: Final published version
Serval ID
serval:BIB_36B69997AF74
Type
Article: article from journal or magazin.
Publication sub-type
Review (review): journal as complete as possible of one specific subject, written based on exhaustive analyses from published work.
Collection
Publications
Institution
Title
Analysis, Isolation, and Activation of Antigen-Specific CD4 + and CD8+ T Cells by Soluble MHC-Peptide Complexes
Journal
Frontiers In Immunology
Author(s)
Schmidt J., Dojcinovic D., Guillaume P., Luescher I.
ISSN
1664-3224 (Electronic)
ISSN-L
1664-3224
Publication state
Published
Issued date
2013
Peer-reviewed
Oui
Volume
4
Pages
218
Language
english
Notes
Publication types: Immunology ; review-article Identifiant PubMed Central: PMC3726995
Abstract
T cells constitute the core of adaptive cellular immunity and protect higher organisms against pathogen infections and cancer. Monitoring of disease progression as well as prophylactic or therapeutic vaccines and immunotherapies call for conclusive detection, analysis, and sorting of antigen-specific T cells. This is possible by means of soluble recombinant ligands for T cells, i.e., MHC class I-peptide (pMHC I) complexes for CD8(+) T cells and MHC class II-peptide (pMHC II) complexes for CD4(+) T cells and flow cytometry. Here we review major developments in the development of pMHC staining reagents and their diverse applications and discuss perspectives of their use for basic and clinical investigations.
Keywords
T cells, T cell receptor, tetramers, MHC, flow cytometry, coreceptor
Pubmed
Web of science
Open Access
Yes
Create date
11/07/2016 10:05
Last modification date
20/08/2019 13:24
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