Limited access to antigen drives generation of early B cell memory while restraining the plasmablast response.

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Accès restreint UNIL
Etat: Public
Version: Final published version
Licence: Non spécifiée
ID Serval
serval:BIB_35B9E7F5BCE4
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Limited access to antigen drives generation of early B cell memory while restraining the plasmablast response.
Périodique
Immunity
Auteur⸱e⸱s
Glaros V., Rauschmeier R., Artemov A.V., Reinhardt A., Ols S., Emmanouilidi A., Gustafsson C., You Y., Mirabello C., Björklund Å.K., Perez L., King N.P., Månsson R., Angeletti D., Loré K., Adameyko I., Busslinger M., Kreslavsky T.
ISSN
1097-4180 (Electronic)
ISSN-L
1074-7613
Statut éditorial
Publié
Date de publication
14/09/2021
Peer-reviewed
Oui
Volume
54
Numéro
9
Pages
2005-2023.e10
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: ppublish
Résumé
Cell fate decisions during early B cell activation determine the outcome of responses to pathogens and vaccines. We examined the early B cell response to T-dependent antigen in mice by single-cell RNA sequencing. Early after immunization, a homogeneous population of activated precursors (APs) gave rise to a transient wave of plasmablasts (PBs), followed a day later by the emergence of germinal center B cells (GCBCs). Most APs rapidly exited the cell cycle, giving rise to non-GC-derived early memory B cells (eMBCs) that retained an AP-like transcriptional profile. Rapid decline of antigen availability controlled these events; provision of excess antigen precluded cell cycle exit and induced a new wave of PBs. Fate mapping revealed a prominent contribution of eMBCs to the MBC pool. Quiescent cells with an MBC phenotype dominated the early response to immunization in primates. A reservoir of APs/eMBCs may enable rapid readjustment of the immune response when failure to contain a threat is manifested by increased antigen availability.
Mots-clé
B cells, cell fate decisions, germinal center B cells, germinal centers, humoral immune response, immunological memory, memory B cells, plasma cells, plasmablasts, scRNA-seq
Pubmed
Web of science
Open Access
Oui
Création de la notice
16/09/2021 7:38
Dernière modification de la notice
20/07/2022 5:38
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