Limited access to antigen drives generation of early B cell memory while restraining the plasmablast response.

Details

Serval ID
serval:BIB_35B9E7F5BCE4
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Limited access to antigen drives generation of early B cell memory while restraining the plasmablast response.
Journal
Immunity
Author(s)
Glaros V., Rauschmeier R., Artemov A.V., Reinhardt A., Ols S., Emmanouilidi A., Gustafsson C., You Y., Mirabello C., Björklund Å.K., Perez L., King N.P., Månsson R., Angeletti D., Loré K., Adameyko I., Busslinger M., Kreslavsky T.
ISSN
1097-4180 (Electronic)
ISSN-L
1074-7613
Publication state
Published
Issued date
14/09/2021
Peer-reviewed
Oui
Volume
54
Number
9
Pages
2005-2023.e10
Language
english
Notes
Publication types: Journal Article
Publication Status: ppublish
Abstract
Cell fate decisions during early B cell activation determine the outcome of responses to pathogens and vaccines. We examined the early B cell response to T-dependent antigen in mice by single-cell RNA sequencing. Early after immunization, a homogeneous population of activated precursors (APs) gave rise to a transient wave of plasmablasts (PBs), followed a day later by the emergence of germinal center B cells (GCBCs). Most APs rapidly exited the cell cycle, giving rise to non-GC-derived early memory B cells (eMBCs) that retained an AP-like transcriptional profile. Rapid decline of antigen availability controlled these events; provision of excess antigen precluded cell cycle exit and induced a new wave of PBs. Fate mapping revealed a prominent contribution of eMBCs to the MBC pool. Quiescent cells with an MBC phenotype dominated the early response to immunization in primates. A reservoir of APs/eMBCs may enable rapid readjustment of the immune response when failure to contain a threat is manifested by increased antigen availability.
Keywords
B cells, cell fate decisions, germinal center B cells, germinal centers, humoral immune response, immunological memory, memory B cells, plasma cells, plasmablasts, scRNA-seq
Pubmed
Web of science
Open Access
Yes
Create date
16/09/2021 7:38
Last modification date
02/10/2021 5:36
Usage data