Extrasynaptic acetylcholine signaling through a muscarinic receptor regulates cell migration.

Détails

ID Serval
serval:BIB_34CE97731E71
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Extrasynaptic acetylcholine signaling through a muscarinic receptor regulates cell migration.
Périodique
Proceedings of the National Academy of Sciences of the United States of America
Auteur(s)
Kato M., Kolotuev I., Cunha A., Gharib S., Sternberg P.W.
ISSN
1091-6490 (Electronic)
ISSN-L
0027-8424
Statut éditorial
Publié
Date de publication
05/01/2021
Peer-reviewed
Oui
Volume
118
Numéro
1
Pages
e1904338118
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
Acetylcholine (ACh) promotes various cell migrations in vitro, but there are few investigations into this nonsynaptic role of ACh signaling in vivo. Here we investigate the function of a muscarinic receptor on an epithelial cell migration in Caenorhabditis elegans We show that the migratory gonad leader cell, the linker cell (LC), uses an M1/M3/M5-like muscarinic ACh receptor GAR-3 to receive extrasynaptic ACh signaling from cholinergic neurons for its migration. Either the loss of the GAR-3 receptor in the LC or the inhibition of ACh release from cholinergic neurons resulted in migratory path defects. The overactivation of the GAR-3 muscarinic receptor caused the LC to reverse its orientation through its downstream effectors Gαq/egl-30, PLCβ/egl-8, and TRIO/unc-73 This reversal response only occurred in the fourth larval stage, which corresponds to the developmental time when the GAR-3::yellow fluorescent protein receptor in the membrane relocalizes from a uniform to an asymmetric distribution. These findings suggest a role for the GAR-3 muscarinic receptor in determining the direction of LC migration.
Mots-clé
Acetylcholine/metabolism, Acetylcholine/physiology, Animals, Caenorhabditis elegans/metabolism, Caenorhabditis elegans Proteins/metabolism, Caenorhabditis elegans Proteins/physiology, Cell Movement/physiology, Epithelial Cells/metabolism, Muscle Contraction/physiology, Presynaptic Terminals/metabolism, Receptors, Muscarinic/metabolism, Receptors, Muscarinic/physiology, Signal Transduction, acetylcholine, cell migration, muscarinic receptor
Pubmed
Web of science
Création de la notice
23/02/2021 14:53
Dernière modification de la notice
25/05/2021 6:34
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