Extrasynaptic acetylcholine signaling through a muscarinic receptor regulates cell migration.

Details

Serval ID
serval:BIB_34CE97731E71
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Extrasynaptic acetylcholine signaling through a muscarinic receptor regulates cell migration.
Journal
Proceedings of the National Academy of Sciences of the United States of America
Author(s)
Kato M., Kolotuev I., Cunha A., Gharib S., Sternberg P.W.
ISSN
1091-6490 (Electronic)
ISSN-L
0027-8424
Publication state
Published
Issued date
05/01/2021
Peer-reviewed
Oui
Volume
118
Number
1
Pages
e1904338118
Language
english
Notes
Publication types: Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
Acetylcholine (ACh) promotes various cell migrations in vitro, but there are few investigations into this nonsynaptic role of ACh signaling in vivo. Here we investigate the function of a muscarinic receptor on an epithelial cell migration in Caenorhabditis elegans We show that the migratory gonad leader cell, the linker cell (LC), uses an M1/M3/M5-like muscarinic ACh receptor GAR-3 to receive extrasynaptic ACh signaling from cholinergic neurons for its migration. Either the loss of the GAR-3 receptor in the LC or the inhibition of ACh release from cholinergic neurons resulted in migratory path defects. The overactivation of the GAR-3 muscarinic receptor caused the LC to reverse its orientation through its downstream effectors Gαq/egl-30, PLCβ/egl-8, and TRIO/unc-73 This reversal response only occurred in the fourth larval stage, which corresponds to the developmental time when the GAR-3::yellow fluorescent protein receptor in the membrane relocalizes from a uniform to an asymmetric distribution. These findings suggest a role for the GAR-3 muscarinic receptor in determining the direction of LC migration.
Keywords
Acetylcholine/metabolism, Acetylcholine/physiology, Animals, Caenorhabditis elegans/metabolism, Caenorhabditis elegans Proteins/metabolism, Caenorhabditis elegans Proteins/physiology, Cell Movement/physiology, Epithelial Cells/metabolism, Muscle Contraction/physiology, Presynaptic Terminals/metabolism, Receptors, Muscarinic/metabolism, Receptors, Muscarinic/physiology, Signal Transduction, acetylcholine, cell migration, muscarinic receptor
Pubmed
Web of science
Create date
23/02/2021 14:53
Last modification date
25/05/2021 6:34
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