Telomerase activity and human telomerase reverse transcriptase mRNA expression in soft tissue tumors: correlation with grade, histology, and proliferative activity.

Détails

ID Serval
serval:BIB_3453272520E0
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Telomerase activity and human telomerase reverse transcriptase mRNA expression in soft tissue tumors: correlation with grade, histology, and proliferative activity.
Périodique
Cancer Research
Auteur(s)
Yan P., Coindre J.M., Benhattar J., Bosman F.T., Guillou L.
ISSN
0008-5472 (Print)
ISSN-L
0008-5472
Statut éditorial
Publié
Date de publication
1999
Volume
59
Numéro
13
Pages
3166-3170
Langue
anglais
Résumé
Telomerase activity (TA) is detected in most human cancers but, with few exceptions, not in normal somatic cells. Little is known about TA in soft tissue tumors. We have examined a series of benign and malignant soft tissue tumors for TA using the telomerase repeat amplification protocol assay. Analysis of the expression of the human telomerase reverse transcriptase was also carried out using RT-PCR. TA was undetectable in benign lesions (15 of 15) and low-grade sarcomas (6 of 6) and was detectable in 50% (19 of 38) of intermediate-/high-grade sarcomas. Although the presence of TA in soft tissue tumors is synonymous with malignancy, it is neither a reliable method in making the distinction between reactive/benign and malignant (especially low-grade) lesions nor a reliable marker of tumor aggressiveness. Leiomyosarcomas and storiform/pleomorphic malignant fibrous histiocytomas rarely showed TA, irrespective of their grade. A strong correlation between human telomerase reverse transcriptase mRNA expression and TA was observed, supporting the close relationship between both parameters. No significant relationship was observed between proliferative activity (as assessed by MIB-1 immunolabeling) and TA. We verified that the absence of telomerase expression was not due to the presence of telomerase inhibitors and therefore alternative mechanism(s) for cell immortalization, yet to be determined, seem to be involved in the development and/or maintenance of some soft tissue sarcomas.
Mots-clé
Gene Expression Regulation, Neoplastic, Humans, RNA, Messenger/genetics, RNA-Directed DNA Polymerase/genetics, RNA-Directed DNA Polymerase/metabolism, Reverse Transcriptase Polymerase Chain Reaction, Sarcoma/enzymology, Sarcoma/genetics, Soft Tissue Neoplasms/enzymology, Soft Tissue Neoplasms/genetics, Telomerase/genetics, Telomerase/metabolism, Transcription, Genetic
Pubmed
Web of science
Création de la notice
03/09/2011 20:50
Dernière modification de la notice
20/08/2019 14:20
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