Telomerase activity and human telomerase reverse transcriptase mRNA expression in soft tissue tumors: correlation with grade, histology, and proliferative activity.

Details

Serval ID
serval:BIB_3453272520E0
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Telomerase activity and human telomerase reverse transcriptase mRNA expression in soft tissue tumors: correlation with grade, histology, and proliferative activity.
Journal
Cancer Research
Author(s)
Yan P., Coindre J.M., Benhattar J., Bosman F.T., Guillou L.
ISSN
0008-5472 (Print)
ISSN-L
0008-5472
Publication state
Published
Issued date
1999
Volume
59
Number
13
Pages
3166-3170
Language
english
Abstract
Telomerase activity (TA) is detected in most human cancers but, with few exceptions, not in normal somatic cells. Little is known about TA in soft tissue tumors. We have examined a series of benign and malignant soft tissue tumors for TA using the telomerase repeat amplification protocol assay. Analysis of the expression of the human telomerase reverse transcriptase was also carried out using RT-PCR. TA was undetectable in benign lesions (15 of 15) and low-grade sarcomas (6 of 6) and was detectable in 50% (19 of 38) of intermediate-/high-grade sarcomas. Although the presence of TA in soft tissue tumors is synonymous with malignancy, it is neither a reliable method in making the distinction between reactive/benign and malignant (especially low-grade) lesions nor a reliable marker of tumor aggressiveness. Leiomyosarcomas and storiform/pleomorphic malignant fibrous histiocytomas rarely showed TA, irrespective of their grade. A strong correlation between human telomerase reverse transcriptase mRNA expression and TA was observed, supporting the close relationship between both parameters. No significant relationship was observed between proliferative activity (as assessed by MIB-1 immunolabeling) and TA. We verified that the absence of telomerase expression was not due to the presence of telomerase inhibitors and therefore alternative mechanism(s) for cell immortalization, yet to be determined, seem to be involved in the development and/or maintenance of some soft tissue sarcomas.
Keywords
Gene Expression Regulation, Neoplastic, Humans, RNA, Messenger/genetics, RNA-Directed DNA Polymerase/genetics, RNA-Directed DNA Polymerase/metabolism, Reverse Transcriptase Polymerase Chain Reaction, Sarcoma/enzymology, Sarcoma/genetics, Soft Tissue Neoplasms/enzymology, Soft Tissue Neoplasms/genetics, Telomerase/genetics, Telomerase/metabolism, Transcription, Genetic
Pubmed
Web of science
Create date
03/09/2011 19:50
Last modification date
20/08/2019 13:20
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