Evaluation of a PCR-RFLP assay for the identification of dermatophytes in situ

Détails

ID Serval
serval:BIB_32586B599882
Type
Actes de conférence (partie): contribution originale à la littérature scientifique, publiée à l'occasion de conférences scientifiques, dans un ouvrage de compte-rendu (proceedings), ou dans l'édition spéciale d'un journal reconnu (conference proceedings).
Sous-type
Abstract (résumé de présentation): article court qui reprend les éléments essentiels présentés à l'occasion d'une conférence scientifique dans un poster ou lors d'une intervention orale.
Collection
Publications
Institution
Titre
Evaluation of a PCR-RFLP assay for the identification of dermatophytes in situ
Titre de la conférence
Annual Congress of the French Speaking Society for Dermatological Research
Auteur(s)
Verrier J., Bontems O., Fratti M., Salamin K., Monod M.
Adresse
Besançon, France, June 23-24, 2011
ISBN
0022-202X
Statut éditorial
Publié
Date de publication
2011
Peer-reviewed
Oui
Volume
131
Série
Journal of Investigative Dermatology
Pages
2150
Langue
anglais
Notes
Publication type : Meeting Abstract
Résumé
Dermatophytes are the main cause of superficial mycoses. These fungi have the capacity to invade keratinized tissue of humans or animals to produce infections that are generally restricted to the corneocytes of the skin, hair, and nails. Nevertheless, it is common to obtain negative results from fungal cultures of dermatological specimens where direct mycological examination showed fungal elements (30-40%). However, correct identification of the isolated dermatophytes from Tinea is important to choose the appropriate treatment. Therefore, we aim to develop a rapid polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assay based on 28S rDNA that is able to identify dermatophytes species in positive dermatological samples. PCR-RFLP identification of dermatophytes in skin or hair allowed validation of the results obtained in culture. It was also possible to identify the infectious dermatophytes when direct hair/ skin mycological examination showed fungal elements, but negative results were obtained from fungal culture. As a conclusion, PCR methods may provide significant benefits in the rapid diagnosis of Tinea. First, there is an increase in sensitivity of dermatophytes identification when enough material is available. Secondly, identification of the infecting agent can be obtained in 24 hours with PCR-RFLP or sequencing, whereas results from fungal cultures can take 2-3 weeks.
Mots-clé
,
Web of science
Création de la notice
07/10/2011 10:33
Dernière modification de la notice
20/08/2019 14:17
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