Evaluation of a PCR-RFLP assay for the identification of dermatophytes in situ

Details

Serval ID
serval:BIB_32586B599882
Type
Inproceedings: an article in a conference proceedings.
Publication sub-type
Abstract (Abstract): shot summary in a article that contain essentials elements presented during a scientific conference, lecture or from a poster.
Collection
Publications
Institution
Title
Evaluation of a PCR-RFLP assay for the identification of dermatophytes in situ
Title of the conference
Annual Congress of the French Speaking Society for Dermatological Research
Author(s)
Verrier J., Bontems O., Fratti M., Salamin K., Monod M.
Address
Besançon, France, June 23-24, 2011
ISBN
0022-202X
Publication state
Published
Issued date
2011
Peer-reviewed
Oui
Volume
131
Series
Journal of Investigative Dermatology
Pages
2150
Language
english
Notes
Publication type : Meeting Abstract
Abstract
Dermatophytes are the main cause of superficial mycoses. These fungi have the capacity to invade keratinized tissue of humans or animals to produce infections that are generally restricted to the corneocytes of the skin, hair, and nails. Nevertheless, it is common to obtain negative results from fungal cultures of dermatological specimens where direct mycological examination showed fungal elements (30-40%). However, correct identification of the isolated dermatophytes from Tinea is important to choose the appropriate treatment. Therefore, we aim to develop a rapid polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assay based on 28S rDNA that is able to identify dermatophytes species in positive dermatological samples. PCR-RFLP identification of dermatophytes in skin or hair allowed validation of the results obtained in culture. It was also possible to identify the infectious dermatophytes when direct hair/ skin mycological examination showed fungal elements, but negative results were obtained from fungal culture. As a conclusion, PCR methods may provide significant benefits in the rapid diagnosis of Tinea. First, there is an increase in sensitivity of dermatophytes identification when enough material is available. Secondly, identification of the infecting agent can be obtained in 24 hours with PCR-RFLP or sequencing, whereas results from fungal cultures can take 2-3 weeks.
Keywords
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Web of science
Create date
07/10/2011 9:33
Last modification date
20/08/2019 13:17
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