Benchmarking various green fluorescent protein variants in Bacillus subtilis, Streptococcus pneumoniae, and Lactococcus lactis for live cell imaging.

Détails

ID Serval
serval:BIB_31E48788E5EC
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Benchmarking various green fluorescent protein variants in Bacillus subtilis, Streptococcus pneumoniae, and Lactococcus lactis for live cell imaging.
Périodique
Applied and Environmental Microbiology
Auteur⸱e⸱s
Overkamp W., Beilharz K., Detert Oude Weme R., Solopova A., Karsens H., Kovács Á., Kok J., Kuipers O.P., Veening J.W.
ISSN
1098-5336 (Electronic)
ISSN-L
0099-2240
Statut éditorial
Publié
Date de publication
2013
Volume
79
Numéro
20
Pages
6481-6490
Langue
anglais
Résumé
Green fluorescent protein (GFP) offers efficient ways of visualizing promoter activity and protein localization in vivo, and many different variants are currently available to study bacterial cell biology. Which of these variants is best suited for a certain bacterial strain, goal, or experimental condition is not clear. Here, we have designed and constructed two "superfolder" GFPs with codon adaptation specifically for Bacillus subtilis and Streptococcus pneumoniae and have benchmarked them against five other previously available variants of GFP in B. subtilis, S. pneumoniae, and Lactococcus lactis, using promoter-gfp fusions. Surprisingly, the best-performing GFP under our experimental conditions in B. subtilis was the one codon optimized for S. pneumoniae and vice versa. The data and tools described in this study will be useful for cell biology studies in low-GC-rich Gram-positive bacteria.
Mots-clé
Bacillus subtilis/chemistry, Bacillus subtilis/genetics, Benchmarking, DNA, Bacterial/chemistry, DNA, Bacterial/genetics, Green Fluorescent Proteins/analysis, Green Fluorescent Proteins/genetics, Lactococcus lactis/chemistry, Lactococcus lactis/genetics, Microscopy, Fluorescence/methods, Molecular Sequence Data, Sequence Analysis, DNA, Staining and Labeling/methods, Streptococcus pneumoniae/chemistry, Streptococcus pneumoniae/genetics
Pubmed
Web of science
Open Access
Oui
Création de la notice
11/10/2016 15:32
Dernière modification de la notice
20/08/2019 13:17
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