Investigation of miR9-1, miR9-2 and miR9-3 Methylation in Hodgkin Lymphoma.
Détails
ID Serval
serval:BIB_319DE71E7B24
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Investigation of miR9-1, miR9-2 and miR9-3 Methylation in Hodgkin Lymphoma.
Périodique
Pathobiology
ISSN
1423-0291 (Electronic)
ISSN-L
1015-2008
Statut éditorial
Publié
Date de publication
2015
Peer-reviewed
Oui
Volume
82
Numéro
5
Pages
195-202
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: ppublish
Publication Status: ppublish
Résumé
miR9 is an important tumor suppressor microRNA regulated by DNA methylation in various types of cancers.
We analyzed the methylation status of the 3 members of the miR9 family in 58 cases of Hodgkin lymphoma (HL) in comparison to 15 reactive lymph nodes. We also assessed the relationships between miR9 methylation and Epstein-Barr virus (EBV) infection and several clinicopathological parameters.
We found that 84.5% of HL cases had a methylation in at least 1 of the 3 loci of miR9, whereas none of the nontumoral samples was methylated. The highest rate of methylation was found in miR9-2 (5q14.3) in 74.1% of the HL cases, followed by miR9-3 (15q26.1) in 56.9% and miR9-1 (1q22) in only 8.6% (p < 0.001). The promoter methylation of miR9-3 was more frequent in patients older than 15 years than in children (p = 0.02) and among women rather than men (p = 0.02). However, no significant correlation was found between miR9 methylation and EBV infection.
These results indicate that miR9 methylation, especially miR9-2, is a frequent event in HL and may be involved in HL pathogenesis, irrespective of EBV infection.
We analyzed the methylation status of the 3 members of the miR9 family in 58 cases of Hodgkin lymphoma (HL) in comparison to 15 reactive lymph nodes. We also assessed the relationships between miR9 methylation and Epstein-Barr virus (EBV) infection and several clinicopathological parameters.
We found that 84.5% of HL cases had a methylation in at least 1 of the 3 loci of miR9, whereas none of the nontumoral samples was methylated. The highest rate of methylation was found in miR9-2 (5q14.3) in 74.1% of the HL cases, followed by miR9-3 (15q26.1) in 56.9% and miR9-1 (1q22) in only 8.6% (p < 0.001). The promoter methylation of miR9-3 was more frequent in patients older than 15 years than in children (p = 0.02) and among women rather than men (p = 0.02). However, no significant correlation was found between miR9 methylation and EBV infection.
These results indicate that miR9 methylation, especially miR9-2, is a frequent event in HL and may be involved in HL pathogenesis, irrespective of EBV infection.
Mots-clé
Adolescent, Adult, Age Factors, Aged, Aged, 80 and over, Child, Child, Preschool, DNA Methylation, Epstein-Barr Virus Infections/complications, Epstein-Barr Virus Infections/genetics, Epstein-Barr Virus Infections/virology, Female, Hodgkin Disease/genetics, Hodgkin Disease/pathology, Hodgkin Disease/virology, Humans, Male, MicroRNAs/genetics, Middle Aged, Promoter Regions, Genetic, Sex Factors, Young Adult
Pubmed
Web of science
Création de la notice
17/10/2023 8:54
Dernière modification de la notice
20/10/2023 7:10
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