Investigation of miR9-1, miR9-2 and miR9-3 Methylation in Hodgkin Lymphoma.
Details
Serval ID
serval:BIB_319DE71E7B24
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Investigation of miR9-1, miR9-2 and miR9-3 Methylation in Hodgkin Lymphoma.
Journal
Pathobiology
ISSN
1423-0291 (Electronic)
ISSN-L
1015-2008
Publication state
Published
Issued date
2015
Peer-reviewed
Oui
Volume
82
Number
5
Pages
195-202
Language
english
Notes
Publication types: Journal Article
Publication Status: ppublish
Publication Status: ppublish
Abstract
miR9 is an important tumor suppressor microRNA regulated by DNA methylation in various types of cancers.
We analyzed the methylation status of the 3 members of the miR9 family in 58 cases of Hodgkin lymphoma (HL) in comparison to 15 reactive lymph nodes. We also assessed the relationships between miR9 methylation and Epstein-Barr virus (EBV) infection and several clinicopathological parameters.
We found that 84.5% of HL cases had a methylation in at least 1 of the 3 loci of miR9, whereas none of the nontumoral samples was methylated. The highest rate of methylation was found in miR9-2 (5q14.3) in 74.1% of the HL cases, followed by miR9-3 (15q26.1) in 56.9% and miR9-1 (1q22) in only 8.6% (p < 0.001). The promoter methylation of miR9-3 was more frequent in patients older than 15 years than in children (p = 0.02) and among women rather than men (p = 0.02). However, no significant correlation was found between miR9 methylation and EBV infection.
These results indicate that miR9 methylation, especially miR9-2, is a frequent event in HL and may be involved in HL pathogenesis, irrespective of EBV infection.
We analyzed the methylation status of the 3 members of the miR9 family in 58 cases of Hodgkin lymphoma (HL) in comparison to 15 reactive lymph nodes. We also assessed the relationships between miR9 methylation and Epstein-Barr virus (EBV) infection and several clinicopathological parameters.
We found that 84.5% of HL cases had a methylation in at least 1 of the 3 loci of miR9, whereas none of the nontumoral samples was methylated. The highest rate of methylation was found in miR9-2 (5q14.3) in 74.1% of the HL cases, followed by miR9-3 (15q26.1) in 56.9% and miR9-1 (1q22) in only 8.6% (p < 0.001). The promoter methylation of miR9-3 was more frequent in patients older than 15 years than in children (p = 0.02) and among women rather than men (p = 0.02). However, no significant correlation was found between miR9 methylation and EBV infection.
These results indicate that miR9 methylation, especially miR9-2, is a frequent event in HL and may be involved in HL pathogenesis, irrespective of EBV infection.
Keywords
Adolescent, Adult, Age Factors, Aged, Aged, 80 and over, Child, Child, Preschool, DNA Methylation, Epstein-Barr Virus Infections/complications, Epstein-Barr Virus Infections/genetics, Epstein-Barr Virus Infections/virology, Female, Hodgkin Disease/genetics, Hodgkin Disease/pathology, Hodgkin Disease/virology, Humans, Male, MicroRNAs/genetics, Middle Aged, Promoter Regions, Genetic, Sex Factors, Young Adult
Pubmed
Web of science
Create date
17/10/2023 8:54
Last modification date
20/10/2023 7:10
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