In vivo measurement of glucose utilization in rats using a beta-microprobe: direct comparison with autoradiography.

Détails

ID Serval
serval:BIB_31541
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
In vivo measurement of glucose utilization in rats using a beta-microprobe: direct comparison with autoradiography.
Périodique
Journal of Cerebral Blood Flow and Metabolism
Auteur⸱e⸱s
Millet P., Sallanon M.M., Petit J.M., Charnay Y., Vallet P., Morel C., Cespuglio R., Magistretti P.J., Ibáñez V.
ISSN
0271-678X
Statut éditorial
Publié
Date de publication
2004
Volume
24
Numéro
9
Pages
1015-1024
Langue
anglais
Notes
Publication types: Comparative Study ; Journal Article ; Research Support, Non-U.S. Gov't Publication Status: ppublish
Résumé
A new beta-microprobe (betaP) has been used to locally measure the time-concentration curve of a radiolabeled substance. The betaP, analogous to positron emission tomography methodology, is useful for in vivo animal studies because it can acquire time-concentration curves with high temporal and spatial resolution. Using [18F]fluoro-2-deoxy-D-glucose and betaP, we evaluated the reliability of the biologic parameters and we compared this method with the [14C]2-deoxy-D-glucose autoradiography. BetaP time-concentration curves in three regions of the brain were obtained from 24 rats. Four kinetic parameters (K1-k4) were estimated from 60-minute experimental periods using a three-compartment model. Best fits were obtained when the vascular fraction (Fv) was estimated simultaneously with the four kinetic parameters (K1-k4). The mean estimated Fv values were about 5.5% for the frontal cortex regions and 8.0% for the cerebellum. Correlation coefficients higher than 0.830 were observed between regional cerebral metabolic rates for glucose (rCMRglc) values obtained by betaP and autoradiography. In addition, the betaP-derived input function was similar to that obtained by manual sampling. Our findings show that reliable rCMRglc values can be obtained using betaP.
Mots-clé
Animals, Autoradiography, Brain/metabolism, Carbon Radioisotopes/metabolism, Deoxyglucose/metabolism, Diagnostic Imaging/methods, Dose-Response Relationship, Drug, Fluorine Radioisotopes/metabolism, Fluorodeoxyglucose F18/metabolism, Glucose/metabolism, Male, Models, Neurological, Rats, Rats, Sprague-Dawley, Time Factors
Pubmed
Web of science
Création de la notice
19/11/2007 12:30
Dernière modification de la notice
20/08/2019 13:16
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