In vivo measurement of glucose utilization in rats using a beta-microprobe: direct comparison with autoradiography.

Details

Serval ID
serval:BIB_31541
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
In vivo measurement of glucose utilization in rats using a beta-microprobe: direct comparison with autoradiography.
Journal
Journal of Cerebral Blood Flow and Metabolism
Author(s)
Millet P., Sallanon M.M., Petit J.M., Charnay Y., Vallet P., Morel C., Cespuglio R., Magistretti P.J., Ibáñez V.
ISSN
0271-678X
Publication state
Published
Issued date
2004
Volume
24
Number
9
Pages
1015-1024
Language
english
Notes
Publication types: Comparative Study ; Journal Article ; Research Support, Non-U.S. Gov't Publication Status: ppublish
Abstract
A new beta-microprobe (betaP) has been used to locally measure the time-concentration curve of a radiolabeled substance. The betaP, analogous to positron emission tomography methodology, is useful for in vivo animal studies because it can acquire time-concentration curves with high temporal and spatial resolution. Using [18F]fluoro-2-deoxy-D-glucose and betaP, we evaluated the reliability of the biologic parameters and we compared this method with the [14C]2-deoxy-D-glucose autoradiography. BetaP time-concentration curves in three regions of the brain were obtained from 24 rats. Four kinetic parameters (K1-k4) were estimated from 60-minute experimental periods using a three-compartment model. Best fits were obtained when the vascular fraction (Fv) was estimated simultaneously with the four kinetic parameters (K1-k4). The mean estimated Fv values were about 5.5% for the frontal cortex regions and 8.0% for the cerebellum. Correlation coefficients higher than 0.830 were observed between regional cerebral metabolic rates for glucose (rCMRglc) values obtained by betaP and autoradiography. In addition, the betaP-derived input function was similar to that obtained by manual sampling. Our findings show that reliable rCMRglc values can be obtained using betaP.
Keywords
Animals, Autoradiography, Brain/metabolism, Carbon Radioisotopes/metabolism, Deoxyglucose/metabolism, Diagnostic Imaging/methods, Dose-Response Relationship, Drug, Fluorine Radioisotopes/metabolism, Fluorodeoxyglucose F18/metabolism, Glucose/metabolism, Male, Models, Neurological, Rats, Rats, Sprague-Dawley, Time Factors
Pubmed
Web of science
Create date
19/11/2007 12:30
Last modification date
20/08/2019 13:16
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