Quantitative intrinsic auto-cathodoluminescence can resolve spectral signatures of tissue-isolated collagen extracellular matrix.

Détails

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Etat: Public
Version: Final published version
Licence: CC BY 4.0
ID Serval
serval:BIB_2BCA39A08DA4
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Quantitative intrinsic auto-cathodoluminescence can resolve spectral signatures of tissue-isolated collagen extracellular matrix.
Périodique
Communications biology
Auteur(s)
Zielinski M.S., Vardar E., Vythilingam G., Engelhardt E.M., Hubbell J.A., Frey P., Larsson H.M.
ISSN
2399-3642 (Electronic)
ISSN-L
2399-3642
Statut éditorial
Publié
Date de publication
2019
Peer-reviewed
Oui
Volume
2
Pages
69
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: epublish
Résumé
By analyzing isolated collagen gel samples, we demonstrated in situ detection of spectrally deconvoluted auto-cathodoluminescence signatures of specific molecular content with precise spatial localization over a maximum field of view of 300 µm. Correlation of the secondary electron and the hyperspectral images proved ~40 nm resolution in the optical channel, obtained due to a short carrier diffusion length, suppressed by fibril dimensions and poor electrical conductivity specific to their organic composition. By correlating spectrally analyzed auto-cathodoluminescence with mass spectroscopy data, we differentiated spectral signatures of two extracellular matrices, namely human fibrin complex and rat tail collagen isolate, and uncovered differences in protein distributions of isolated extracellular matrix networks of heterogeneous populations. Furthermore, we demonstrated that cathodoluminescence can monitor the progress of a human cell-mediated remodeling process, where human collagenous matrix was deposited within a rat collagenous matrix. The revealed change of the heterogeneous biological composition was confirmed by mass spectroscopy.
Pubmed
Web of science
Open Access
Oui
Création de la notice
01/04/2019 16:08
Dernière modification de la notice
30/04/2021 6:09
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