Quantitative intrinsic auto-cathodoluminescence can resolve spectral signatures of tissue-isolated collagen extracellular matrix.

Details

Serval ID
serval:BIB_2BCA39A08DA4
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Quantitative intrinsic auto-cathodoluminescence can resolve spectral signatures of tissue-isolated collagen extracellular matrix.
Journal
Communications biology
Author(s)
Zielinski M.S., Vardar E., Vythilingam G., Engelhardt E.M., Hubbell J.A., Frey P., Larsson H.M.
ISSN
2399-3642 (Electronic)
ISSN-L
2399-3642
Publication state
Published
Issued date
2019
Peer-reviewed
Oui
Volume
2
Pages
69
Language
english
Notes
Publication types: Journal Article
Publication Status: epublish
Abstract
By analyzing isolated collagen gel samples, we demonstrated in situ detection of spectrally deconvoluted auto-cathodoluminescence signatures of specific molecular content with precise spatial localization over a maximum field of view of 300 µm. Correlation of the secondary electron and the hyperspectral images proved ~40 nm resolution in the optical channel, obtained due to a short carrier diffusion length, suppressed by fibril dimensions and poor electrical conductivity specific to their organic composition. By correlating spectrally analyzed auto-cathodoluminescence with mass spectroscopy data, we differentiated spectral signatures of two extracellular matrices, namely human fibrin complex and rat tail collagen isolate, and uncovered differences in protein distributions of isolated extracellular matrix networks of heterogeneous populations. Furthermore, we demonstrated that cathodoluminescence can monitor the progress of a human cell-mediated remodeling process, where human collagenous matrix was deposited within a rat collagenous matrix. The revealed change of the heterogeneous biological composition was confirmed by mass spectroscopy.
Pubmed
Web of science
Open Access
Yes
Create date
01/04/2019 16:08
Last modification date
20/08/2019 13:11
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