Typing Listeria monocytogenes: a comparison of random amplification of polymorphic DNA with 5 other methods

Détails

ID Serval
serval:BIB_2B01E1317A4D
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Typing Listeria monocytogenes: a comparison of random amplification of polymorphic DNA with 5 other methods
Périodique
Research in Microbiology
Auteur(s)
Boerlin  P., Bannerman  E., Ischer  F., Rocourt  J., Bille  J.
ISSN
0923-2508
Statut éditorial
Publié
Date de publication
01/1995
Peer-reviewed
Oui
Volume
146
Numéro
1
Pages
35-49
Notes
Comparative Study
In Vitro
Journal Article --- Old month value: Jan
Résumé
One hundred Listeria monocytogenes strains were typed by random amplification of polymorphic DNA (RAPD) with three different primers, and the results were compared with those obtained by serotyping, ribotyping, multilocus enzyme electrophoresis, restriction enzyme analysis and phage typing. The RAPD patterns of strains appear to be stable during epidemics even over periods of several years. Reproducibility of the RAPD patterns was good. The discriminatory power of RAPD typing was the best among all the methods tested. RAPD is therefore a very promising tool in the study of listeriosis epidemiology. However, the problems related to the standardization of the technique first have to be resolved before the wide use of RAPD is possible.
Mots-clé
Bacterial Typing Techniques Electrophoresis, Agar Gel Listeria monocytogenes/*classification/genetics *Nucleic Acid Amplification Techniques Reproducibility of Results
Pubmed
Web of science
Création de la notice
11/02/2008 12:39
Dernière modification de la notice
20/08/2019 13:10
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