Typing Listeria monocytogenes: a comparison of random amplification of polymorphic DNA with 5 other methods

Details

Serval ID
serval:BIB_2B01E1317A4D
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Typing Listeria monocytogenes: a comparison of random amplification of polymorphic DNA with 5 other methods
Journal
Research in Microbiology
Author(s)
Boerlin  P., Bannerman  E., Ischer  F., Rocourt  J., Bille  J.
ISSN
0923-2508
Publication state
Published
Issued date
01/1995
Peer-reviewed
Oui
Volume
146
Number
1
Pages
35-49
Notes
Comparative Study
In Vitro
Journal Article --- Old month value: Jan
Abstract
One hundred Listeria monocytogenes strains were typed by random amplification of polymorphic DNA (RAPD) with three different primers, and the results were compared with those obtained by serotyping, ribotyping, multilocus enzyme electrophoresis, restriction enzyme analysis and phage typing. The RAPD patterns of strains appear to be stable during epidemics even over periods of several years. Reproducibility of the RAPD patterns was good. The discriminatory power of RAPD typing was the best among all the methods tested. RAPD is therefore a very promising tool in the study of listeriosis epidemiology. However, the problems related to the standardization of the technique first have to be resolved before the wide use of RAPD is possible.
Keywords
Bacterial Typing Techniques Electrophoresis, Agar Gel Listeria monocytogenes/*classification/genetics *Nucleic Acid Amplification Techniques Reproducibility of Results
Pubmed
Web of science
Create date
11/02/2008 12:39
Last modification date
20/08/2019 13:10
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