Identification of human synenkephalin-like immunoreactivity in phaeochromocytoma tissue using a novel carboxy-terminal radioimmunoassay

Détails

ID Serval
serval:BIB_29537A2E2BFC
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Identification of human synenkephalin-like immunoreactivity in phaeochromocytoma tissue using a novel carboxy-terminal radioimmunoassay
Périodique
Neuroscience Letters
Auteur⸱e⸱s
Corder  R., Gaillard  R. C., Rossier  J.
ISSN
0304-3940 (Print)
Statut éditorial
Publié
Date de publication
12/1987
Volume
82
Numéro
3
Pages
308-14
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Dec 4
Résumé
An antiserum raised against the synthetic tyrosinylated carboxy-terminal sequence of synenkephalin (Tyr-Glu-Glu-Ser-His-Leu-Leu-Ala) has been used to chromatographically characterize the human synenkephalin-like immunoreactivity extracted from 3 adrenal medullary phaeochromocytomas. Gel filtration chromatography identified in each tumor a single peak of 8 kDa which on subsequent ion-exchange chromatography had the elution characteristics of an acidic polypeptide. These results are compatible with the human synenkephalin sequence predicted from cDNA studies, and indicate that this is the authentic peptide.
Mots-clé
Adrenal Gland Neoplasms/*analysis Chromatography, Gel Chromatography, High Pressure Liquid Chromatography, Ion Exchange Enkephalins/*analysis Humans Peptide Fragments/analysis Pheochromocytoma/*analysis Protein Precursors/*analysis Radioimmunoassay
Pubmed
Web of science
Création de la notice
15/02/2008 16:57
Dernière modification de la notice
20/08/2019 13:09
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