Identification of human synenkephalin-like immunoreactivity in phaeochromocytoma tissue using a novel carboxy-terminal radioimmunoassay

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Serval ID
serval:BIB_29537A2E2BFC
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Identification of human synenkephalin-like immunoreactivity in phaeochromocytoma tissue using a novel carboxy-terminal radioimmunoassay
Journal
Neuroscience Letters
Author(s)
Corder  R., Gaillard  R. C., Rossier  J.
ISSN
0304-3940 (Print)
Publication state
Published
Issued date
12/1987
Volume
82
Number
3
Pages
308-14
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Dec 4
Abstract
An antiserum raised against the synthetic tyrosinylated carboxy-terminal sequence of synenkephalin (Tyr-Glu-Glu-Ser-His-Leu-Leu-Ala) has been used to chromatographically characterize the human synenkephalin-like immunoreactivity extracted from 3 adrenal medullary phaeochromocytomas. Gel filtration chromatography identified in each tumor a single peak of 8 kDa which on subsequent ion-exchange chromatography had the elution characteristics of an acidic polypeptide. These results are compatible with the human synenkephalin sequence predicted from cDNA studies, and indicate that this is the authentic peptide.
Keywords
Adrenal Gland Neoplasms/*analysis Chromatography, Gel Chromatography, High Pressure Liquid Chromatography, Ion Exchange Enkephalins/*analysis Humans Peptide Fragments/analysis Pheochromocytoma/*analysis Protein Precursors/*analysis Radioimmunoassay
Pubmed
Web of science
Create date
15/02/2008 16:57
Last modification date
20/08/2019 13:09
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