Isolation of distinct cDNA clones encoding HLA-DR beta chains by use of an expression assay
Détails
ID Serval
serval:BIB_29266CD2CA3A
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Isolation of distinct cDNA clones encoding HLA-DR beta chains by use of an expression assay
Périodique
Proceedings of the National Academy of Sciences of the United States of America
ISSN
0027-8424
Statut éditorial
Publié
Date de publication
12/1982
Peer-reviewed
Oui
Volume
79
Numéro
23
Pages
7465-9
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Dec
Research Support, Non-U.S. Gov't --- Old month value: Dec
Résumé
cDNA clones encoding different human Ia antigen beta chains were isolated by use of a complementation-expression assay in Xenopus oocytes. The assay was based on two previous findings. First, oocytes injected with mRNA from a human B-cell line express HLA-DR antigen. The three intracellular DR chains are assembled in oocytes and can be immunoprecipitated with anti-DR monoclonal antibodies. Second, we have isolated cDNA clones encoding DR alpha and intermediate chains. In order to identify beta-chain cDNA clones, mRNA was hybrid-selected with pools of cDNA clones, mixed with mRNA for the alpha and intermediate chains, and injected into oocytes. We isolated two distinct clones that could select DR beta-chain mRNA as demonstrated by assembly of the translation product with DR alpha chains and immunoprecipitation with DR-specific monoclonal antibodies. One clone is specific for a beta chain of the DR locus. The other clone, much weaker in its ability to select DR mRNA, encodes another Ia-like beta chain. Full-length cDNA clones corresponding to the DR and Ia-like beta chains were isolated and compared. Cross-hybridization was detectable in the coding regions but not in the 3' untranslated regions. Distinct RNAs homologous to the DR and the Ia-like beta-chain clones were present in B cells but were undetectable in three T-cell lines.
Mots-clé
Base Sequence
Cloning, Molecular
DNA/genetics
*Genes, MHC Class II
HLA-DR Antigens
Humans
Macromolecular Substances
Nucleic Acid Hybridization
RNA, Messenger/genetics
Pubmed
Web of science
Open Access
Oui
Création de la notice
20/01/2008 16:56
Dernière modification de la notice
20/08/2019 14:08