Isolation of distinct cDNA clones encoding HLA-DR beta chains by use of an expression assay
Details
Serval ID
serval:BIB_29266CD2CA3A
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Isolation of distinct cDNA clones encoding HLA-DR beta chains by use of an expression assay
Journal
Proceedings of the National Academy of Sciences of the United States of America
ISSN
0027-8424
Publication state
Published
Issued date
12/1982
Peer-reviewed
Oui
Volume
79
Number
23
Pages
7465-9
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Dec
Research Support, Non-U.S. Gov't --- Old month value: Dec
Abstract
cDNA clones encoding different human Ia antigen beta chains were isolated by use of a complementation-expression assay in Xenopus oocytes. The assay was based on two previous findings. First, oocytes injected with mRNA from a human B-cell line express HLA-DR antigen. The three intracellular DR chains are assembled in oocytes and can be immunoprecipitated with anti-DR monoclonal antibodies. Second, we have isolated cDNA clones encoding DR alpha and intermediate chains. In order to identify beta-chain cDNA clones, mRNA was hybrid-selected with pools of cDNA clones, mixed with mRNA for the alpha and intermediate chains, and injected into oocytes. We isolated two distinct clones that could select DR beta-chain mRNA as demonstrated by assembly of the translation product with DR alpha chains and immunoprecipitation with DR-specific monoclonal antibodies. One clone is specific for a beta chain of the DR locus. The other clone, much weaker in its ability to select DR mRNA, encodes another Ia-like beta chain. Full-length cDNA clones corresponding to the DR and Ia-like beta chains were isolated and compared. Cross-hybridization was detectable in the coding regions but not in the 3' untranslated regions. Distinct RNAs homologous to the DR and the Ia-like beta-chain clones were present in B cells but were undetectable in three T-cell lines.
Keywords
Base Sequence
Cloning, Molecular
DNA/genetics
*Genes, MHC Class II
HLA-DR Antigens
Humans
Macromolecular Substances
Nucleic Acid Hybridization
RNA, Messenger/genetics
Pubmed
Web of science
Open Access
Yes
Create date
20/01/2008 16:56
Last modification date
20/08/2019 14:08