Isolation of distinct cDNA clones encoding HLA-DR beta chains by use of an expression assay

Details

Serval ID
serval:BIB_29266CD2CA3A
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Isolation of distinct cDNA clones encoding HLA-DR beta chains by use of an expression assay
Journal
Proceedings of the National Academy of Sciences of the United States of America
Author(s)
Long  E. O., Wake  C. T., Strubin  M., Gross  N., Accolla  R. S., Carrel  S., Mach  B.
ISSN
0027-8424
Publication state
Published
Issued date
12/1982
Peer-reviewed
Oui
Volume
79
Number
23
Pages
7465-9
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Dec
Abstract
cDNA clones encoding different human Ia antigen beta chains were isolated by use of a complementation-expression assay in Xenopus oocytes. The assay was based on two previous findings. First, oocytes injected with mRNA from a human B-cell line express HLA-DR antigen. The three intracellular DR chains are assembled in oocytes and can be immunoprecipitated with anti-DR monoclonal antibodies. Second, we have isolated cDNA clones encoding DR alpha and intermediate chains. In order to identify beta-chain cDNA clones, mRNA was hybrid-selected with pools of cDNA clones, mixed with mRNA for the alpha and intermediate chains, and injected into oocytes. We isolated two distinct clones that could select DR beta-chain mRNA as demonstrated by assembly of the translation product with DR alpha chains and immunoprecipitation with DR-specific monoclonal antibodies. One clone is specific for a beta chain of the DR locus. The other clone, much weaker in its ability to select DR mRNA, encodes another Ia-like beta chain. Full-length cDNA clones corresponding to the DR and Ia-like beta chains were isolated and compared. Cross-hybridization was detectable in the coding regions but not in the 3' untranslated regions. Distinct RNAs homologous to the DR and the Ia-like beta-chain clones were present in B cells but were undetectable in three T-cell lines.
Keywords
Base Sequence Cloning, Molecular DNA/genetics *Genes, MHC Class II HLA-DR Antigens Humans Macromolecular Substances Nucleic Acid Hybridization RNA, Messenger/genetics
Pubmed
Web of science
Open Access
Yes
Create date
20/01/2008 15:56
Last modification date
20/08/2019 13:08
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