Identification of an expanded population of activated CD4(+) CD25(+) T cells expressing CD45RO and IL-7R in kidney transplant recipients
Détails
ID Serval
serval:BIB_272BEC140FF2
Type
Actes de conférence (partie): contribution originale à la littérature scientifique, publiée à l'occasion de conférences scientifiques, dans un ouvrage de compte-rendu (proceedings), ou dans l'édition spéciale d'un journal reconnu (conference proceedings).
Sous-type
Abstract (résumé de présentation): article court qui reprend les éléments essentiels présentés à l'occasion d'une conférence scientifique dans un poster ou lors d'une intervention orale.
Collection
Publications
Institution
Titre
Identification of an expanded population of activated CD4(+) CD25(+) T cells expressing CD45RO and IL-7R in kidney transplant recipients
Titre de la conférence
13th Congress of the European Society for Organ Transplantation and the 15th Congress of the European Transplant Coordinators Organization
Adresse
Prague, Czech Republic, September 29-October 3, 2007
ISBN
0934-0874
Statut éditorial
Publié
Date de publication
2007
Peer-reviewed
Oui
Volume
20
Série
Transplant International
Pages
153
Langue
anglais
Notes
Publication type : Meeting Abstract
Résumé
Recent studies have shown that CD4+ CD25+ T cells belong to two functionally
different T lymphocytes, i.e. regulatory T cells (Treg) or activated T cells (Tact),
which can be distinguished based on the expression of CD45RO and IL-7R:
Treg (FoxP3+) are CD45RO+ IL-7R- , whereas Tact (FoxP3- ) are CD45RO+ IL-
7R+.
In order to determine if a CD4+ CD25+ CD45RO+ IL-7R+ activated T cell population
might be identified in kidney transplant recipients, we studied 27 healthy
subjects (HS) and 23 kidney recipients, of whom 17 had stable graft function
under standard immunosuppression (IS), 5 had biopsy-proven chronic humoral
rejection (CHR), and one was a stable "tolerant" patient who had discontinued
IS for more than 2 years. Phenotypical analysis by flow cytometry and functional
assays by MLR were performed.
Overall, the Tact population was found to be significantly increased in 87% of
the transplant recipients (mean: 18.8±10.1% of CD4+ CD25+ T cells) compared
to HS (mean: 4.5±2.0%; P<0.0001). In the 5 patients with CHR, this
Tact population was highly expanded (31.3±9.3%; P<0.0001), whereas it
was comparable to HS in the "tolerant" recipient (4.7%). Intermediate levels
(16.0±6.9%; P<0.0001) were found in the 17 stable recipients. In CHR, the
proliferative capacity of the Tact population was found to be 5-fold higher when
stimulated by irradiated donor PBMC as compared to a stimulation by irradiated
3rd party PBMC.
After kidney transplantation, an expanded circulating CD4+ CD25+ T cell population
characterized by the expression of CD45RO and IL-7R was found in
most recipients, particularly in those with CHR. In a patient with long-term
operational tolerance, this Tact population was similar to HS. Measuring circulating
Tact may become a useful monitoring tool after transplantation.
different T lymphocytes, i.e. regulatory T cells (Treg) or activated T cells (Tact),
which can be distinguished based on the expression of CD45RO and IL-7R:
Treg (FoxP3+) are CD45RO+ IL-7R- , whereas Tact (FoxP3- ) are CD45RO+ IL-
7R+.
In order to determine if a CD4+ CD25+ CD45RO+ IL-7R+ activated T cell population
might be identified in kidney transplant recipients, we studied 27 healthy
subjects (HS) and 23 kidney recipients, of whom 17 had stable graft function
under standard immunosuppression (IS), 5 had biopsy-proven chronic humoral
rejection (CHR), and one was a stable "tolerant" patient who had discontinued
IS for more than 2 years. Phenotypical analysis by flow cytometry and functional
assays by MLR were performed.
Overall, the Tact population was found to be significantly increased in 87% of
the transplant recipients (mean: 18.8±10.1% of CD4+ CD25+ T cells) compared
to HS (mean: 4.5±2.0%; P<0.0001). In the 5 patients with CHR, this
Tact population was highly expanded (31.3±9.3%; P<0.0001), whereas it
was comparable to HS in the "tolerant" recipient (4.7%). Intermediate levels
(16.0±6.9%; P<0.0001) were found in the 17 stable recipients. In CHR, the
proliferative capacity of the Tact population was found to be 5-fold higher when
stimulated by irradiated donor PBMC as compared to a stimulation by irradiated
3rd party PBMC.
After kidney transplantation, an expanded circulating CD4+ CD25+ T cell population
characterized by the expression of CD45RO and IL-7R was found in
most recipients, particularly in those with CHR. In a patient with long-term
operational tolerance, this Tact population was similar to HS. Measuring circulating
Tact may become a useful monitoring tool after transplantation.
Mots-clé
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Web of science
Création de la notice
29/12/2010 13:25
Dernière modification de la notice
20/08/2019 14:06
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