Identification of an expanded population of activated CD4(+) CD25(+) T cells expressing CD45RO and IL-7R in kidney transplant recipients
Details
Serval ID
serval:BIB_272BEC140FF2
Type
Inproceedings: an article in a conference proceedings.
Publication sub-type
Abstract (Abstract): shot summary in a article that contain essentials elements presented during a scientific conference, lecture or from a poster.
Collection
Publications
Institution
Title
Identification of an expanded population of activated CD4(+) CD25(+) T cells expressing CD45RO and IL-7R in kidney transplant recipients
Title of the conference
13th Congress of the European Society for Organ Transplantation and the 15th Congress of the European Transplant Coordinators Organization
Address
Prague, Czech Republic, September 29-October 3, 2007
ISBN
0934-0874
Publication state
Published
Issued date
2007
Peer-reviewed
Oui
Volume
20
Series
Transplant International
Pages
153
Language
english
Notes
Publication type : Meeting Abstract
Abstract
Recent studies have shown that CD4+ CD25+ T cells belong to two functionally
different T lymphocytes, i.e. regulatory T cells (Treg) or activated T cells (Tact),
which can be distinguished based on the expression of CD45RO and IL-7R:
Treg (FoxP3+) are CD45RO+ IL-7R- , whereas Tact (FoxP3- ) are CD45RO+ IL-
7R+.
In order to determine if a CD4+ CD25+ CD45RO+ IL-7R+ activated T cell population
might be identified in kidney transplant recipients, we studied 27 healthy
subjects (HS) and 23 kidney recipients, of whom 17 had stable graft function
under standard immunosuppression (IS), 5 had biopsy-proven chronic humoral
rejection (CHR), and one was a stable "tolerant" patient who had discontinued
IS for more than 2 years. Phenotypical analysis by flow cytometry and functional
assays by MLR were performed.
Overall, the Tact population was found to be significantly increased in 87% of
the transplant recipients (mean: 18.8±10.1% of CD4+ CD25+ T cells) compared
to HS (mean: 4.5±2.0%; P<0.0001). In the 5 patients with CHR, this
Tact population was highly expanded (31.3±9.3%; P<0.0001), whereas it
was comparable to HS in the "tolerant" recipient (4.7%). Intermediate levels
(16.0±6.9%; P<0.0001) were found in the 17 stable recipients. In CHR, the
proliferative capacity of the Tact population was found to be 5-fold higher when
stimulated by irradiated donor PBMC as compared to a stimulation by irradiated
3rd party PBMC.
After kidney transplantation, an expanded circulating CD4+ CD25+ T cell population
characterized by the expression of CD45RO and IL-7R was found in
most recipients, particularly in those with CHR. In a patient with long-term
operational tolerance, this Tact population was similar to HS. Measuring circulating
Tact may become a useful monitoring tool after transplantation.
different T lymphocytes, i.e. regulatory T cells (Treg) or activated T cells (Tact),
which can be distinguished based on the expression of CD45RO and IL-7R:
Treg (FoxP3+) are CD45RO+ IL-7R- , whereas Tact (FoxP3- ) are CD45RO+ IL-
7R+.
In order to determine if a CD4+ CD25+ CD45RO+ IL-7R+ activated T cell population
might be identified in kidney transplant recipients, we studied 27 healthy
subjects (HS) and 23 kidney recipients, of whom 17 had stable graft function
under standard immunosuppression (IS), 5 had biopsy-proven chronic humoral
rejection (CHR), and one was a stable "tolerant" patient who had discontinued
IS for more than 2 years. Phenotypical analysis by flow cytometry and functional
assays by MLR were performed.
Overall, the Tact population was found to be significantly increased in 87% of
the transplant recipients (mean: 18.8±10.1% of CD4+ CD25+ T cells) compared
to HS (mean: 4.5±2.0%; P<0.0001). In the 5 patients with CHR, this
Tact population was highly expanded (31.3±9.3%; P<0.0001), whereas it
was comparable to HS in the "tolerant" recipient (4.7%). Intermediate levels
(16.0±6.9%; P<0.0001) were found in the 17 stable recipients. In CHR, the
proliferative capacity of the Tact population was found to be 5-fold higher when
stimulated by irradiated donor PBMC as compared to a stimulation by irradiated
3rd party PBMC.
After kidney transplantation, an expanded circulating CD4+ CD25+ T cell population
characterized by the expression of CD45RO and IL-7R was found in
most recipients, particularly in those with CHR. In a patient with long-term
operational tolerance, this Tact population was similar to HS. Measuring circulating
Tact may become a useful monitoring tool after transplantation.
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Create date
29/12/2010 13:25
Last modification date
20/08/2019 14:06
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