Direct demonstration of the clonogenic potential of every human peripheral blood T cell. Clonal analysis of HLA-DR expression and cytolytic activity.

Détails

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Accès restreint UNIL
Etat: Public
Version: Final published version
Licence: Non spécifiée
ID Serval
serval:BIB_262EBCD084E0
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Direct demonstration of the clonogenic potential of every human peripheral blood T cell. Clonal analysis of HLA-DR expression and cytolytic activity.
Périodique
The Journal of experimental medicine
Auteur⸱e⸱s
Moretta A., Pantaleo G., Moretta L., Cerottini J.C., Mingari M.C.
ISSN
0022-1007
ISSN-L
0022-1007
Statut éditorial
Publié
Date de publication
01/02/1983
Peer-reviewed
Oui
Volume
157
Numéro
2
Pages
743-754
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
In an attempt to determine the clonogenic properties of human peripheral blood T cells, we have developed a limiting dilution microculture system using phytohemagglutinin (PHA) as T cell activator and supernatant from PHA-stimulated spleen cultures as a source of T cell growth factors. The frequencies of cells capable of extensive proliferation under these culture conditions were 0.52-0.73, 0.98-1.11, and less than 0.02 in peripheral blood mononuclear, E-rosette-positive, and E-rosette-negative cell populations, respectively. The clonogenic potential of virtually all T cells was confirmed in experiments using single cells isolated by micromanipulation. Clone size ranged between 5 and 30 X 10(4) cells on day 14 of culture. The same microculture system was used to determine the precursor frequency of all cytolytic T lymphocytes (CTL-P). As assessed by a lectin-dependent 51Cr release assay, the CTL-P frequency in purified T cell populations ranged between 0.30 and 0.34. In comparison, the precursor frequency of T cells capable of lysing K562 target cells was ranging between 0.14 and 0.16. Parallel analysis of individual clonal cultures for both lytic activities showed that 50% of the clones exhibiting lectin-dependent lysis were also active against K562 target cells. All of the proliferating clones expressed HLA-DR antigens, although to a varying degree as assessed by flow cytofluorometry. Given the high cloning efficiency of this culture system, it appears now possible to determine the precursor frequencies of the various classes of functional cells in T cell populations.
Mots-clé
Cells, Cultured, Clone Cells/immunology, Cytotoxicity Tests, Immunologic, HLA-DR Antigens, Hematopoietic Stem Cells/cytology, Hematopoietic Stem Cells/immunology, Histocompatibility Antigens Class II/analysis, Humans, Interleukin-2/pharmacology, Lymphocyte Activation, Phytohemagglutinins/pharmacology, T-Lymphocytes/cytology, T-Lymphocytes/immunology, T-Lymphocytes, Cytotoxic/cytology, T-Lymphocytes, Cytotoxic/immunology
Pubmed
Web of science
Open Access
Oui
Création de la notice
28/01/2008 11:13
Dernière modification de la notice
09/08/2024 14:53
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