Direct demonstration of the clonogenic potential of every human peripheral blood T cell. Clonal analysis of HLA-DR expression and cytolytic activity
Details
Serval ID
serval:BIB_262EBCD084E0
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Direct demonstration of the clonogenic potential of every human peripheral blood T cell. Clonal analysis of HLA-DR expression and cytolytic activity
Journal
Journal of Experimental Medicine
ISSN
0022-1007 (Print)
Publication state
Published
Issued date
02/1983
Volume
157
Number
2
Pages
743-54
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Feb 1
Research Support, Non-U.S. Gov't --- Old month value: Feb 1
Abstract
In an attempt to determine the clonogenic properties of human peripheral blood T cells, we have developed a limiting dilution microculture system using phytohemagglutinin (PHA) as T cell activator and supernatant from PHA-stimulated spleen cultures as a source of T cell growth factors. The frequencies of cells capable of extensive proliferation under these culture conditions were 0.52-0.73, 0.98-1.11, and less than 0.02 in peripheral blood mononuclear, E-rosette-positive, and E-rosette-negative cell populations, respectively. The clonogenic potential of virtually all T cells was confirmed in experiments using single cells isolated by micromanipulation. Clone size ranged between 5 and 30 X 10(4) cells on day 14 of culture. The same microculture system was used to determine the precursor frequency of all cytolytic T lymphocytes (CTL-P). As assessed by a lectin-dependent 51Cr release assay, the CTL-P frequency in purified T cell populations ranged between 0.30 and 0.34. In comparison, the precursor frequency of T cells capable of lysing K562 target cells was ranging between 0.14 and 0.16. Parallel analysis of individual clonal cultures for both lytic activities showed that 50% of the clones exhibiting lectin-dependent lysis were also active against K562 target cells. All of the proliferating clones expressed HLA-DR antigens, although to a varying degree as assessed by flow cytofluorometry. Given the high cloning efficiency of this culture system, it appears now possible to determine the precursor frequencies of the various classes of functional cells in T cell populations.
Keywords
Cells, Cultured
Clone Cells/immunology
Cytotoxicity Tests, Immunologic
HLA-DR Antigens
Hematopoietic Stem Cells/cytology/immunology
Histocompatibility Antigens Class II/*analysis
Humans
Interleukin-2/pharmacology
*Lymphocyte Activation
Phytohemagglutinins/pharmacology
T-Lymphocytes/cytology/*immunology
T-Lymphocytes, Cytotoxic/cytology/*immunology
Pubmed
Web of science
Open Access
Yes
Create date
28/01/2008 12:13
Last modification date
20/08/2019 14:04