Heme oxygenase-1 induction by endogenous nitric oxide: influence of intracellular glutathione.

Détails

ID Serval
serval:BIB_26286
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Heme oxygenase-1 induction by endogenous nitric oxide: influence of intracellular glutathione.
Périodique
FEBS Letters
Auteur⸱e⸱s
André M., Felley-Bosco E.
ISSN
0014-5793
Statut éditorial
Publié
Date de publication
2003
Volume
546
Numéro
2-3
Pages
223-227
Langue
anglais
Résumé
To investigate the influence of glutathione (GSH) on cellular effects of nitric oxide (NO) formation, human colon adenocarcinoma cells were transfected with a vector allowing controlled expression of inducible nitric oxide synthase (iNOS). Protein levels of oxidative stress-sensitive heme oxygenase-1 (HO-1) were analyzed in the presence or absence of GSH depletion using L-buthionine-[S,R]-sulfoximine and iNOS induction. While no effect was observed in the presence of iNOS activity alone, a synergistic effect on HO-1 expression was observed in the presence of iNOS expression and GSH depletion. This effect was prevented by addition of N-methyl-L-arginine. Therefore, targeting of endogenous NO may be modulated by intracellular GSH.
Mots-clé
Base Sequence, DNA Primers, Enzyme Induction, Glutathione, Heme Oxygenase (Decyclizing), Humans, Nitric Oxide, Nitric Oxide Synthase, Nitric Oxide Synthase Type II, Tumor Cells, Cultured
Pubmed
Web of science
Création de la notice
19/11/2007 13:22
Dernière modification de la notice
20/08/2019 14:04
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