Heme oxygenase-1 induction by endogenous nitric oxide: influence of intracellular glutathione.
Détails
ID Serval
serval:BIB_26286
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Heme oxygenase-1 induction by endogenous nitric oxide: influence of intracellular glutathione.
Périodique
FEBS Letters
ISSN
0014-5793
Statut éditorial
Publié
Date de publication
2003
Volume
546
Numéro
2-3
Pages
223-227
Langue
anglais
Résumé
To investigate the influence of glutathione (GSH) on cellular effects of nitric oxide (NO) formation, human colon adenocarcinoma cells were transfected with a vector allowing controlled expression of inducible nitric oxide synthase (iNOS). Protein levels of oxidative stress-sensitive heme oxygenase-1 (HO-1) were analyzed in the presence or absence of GSH depletion using L-buthionine-[S,R]-sulfoximine and iNOS induction. While no effect was observed in the presence of iNOS activity alone, a synergistic effect on HO-1 expression was observed in the presence of iNOS expression and GSH depletion. This effect was prevented by addition of N-methyl-L-arginine. Therefore, targeting of endogenous NO may be modulated by intracellular GSH.
Mots-clé
Base Sequence, DNA Primers, Enzyme Induction, Glutathione, Heme Oxygenase (Decyclizing), Humans, Nitric Oxide, Nitric Oxide Synthase, Nitric Oxide Synthase Type II, Tumor Cells, Cultured
OAI-PMH
Pubmed
Web of science
Création de la notice
19/11/2007 13:22
Dernière modification de la notice
20/08/2019 14:04