Heme oxygenase-1 induction by endogenous nitric oxide: influence of intracellular glutathione.

Details

Serval ID
serval:BIB_26286
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Heme oxygenase-1 induction by endogenous nitric oxide: influence of intracellular glutathione.
Journal
FEBS Letters
Author(s)
André M., Felley-Bosco E.
ISSN
0014-5793
Publication state
Published
Issued date
2003
Volume
546
Number
2-3
Pages
223-227
Language
english
Abstract
To investigate the influence of glutathione (GSH) on cellular effects of nitric oxide (NO) formation, human colon adenocarcinoma cells were transfected with a vector allowing controlled expression of inducible nitric oxide synthase (iNOS). Protein levels of oxidative stress-sensitive heme oxygenase-1 (HO-1) were analyzed in the presence or absence of GSH depletion using L-buthionine-[S,R]-sulfoximine and iNOS induction. While no effect was observed in the presence of iNOS activity alone, a synergistic effect on HO-1 expression was observed in the presence of iNOS expression and GSH depletion. This effect was prevented by addition of N-methyl-L-arginine. Therefore, targeting of endogenous NO may be modulated by intracellular GSH.
Keywords
Base Sequence, DNA Primers, Enzyme Induction, Glutathione, Heme Oxygenase (Decyclizing), Humans, Nitric Oxide, Nitric Oxide Synthase, Nitric Oxide Synthase Type II, Tumor Cells, Cultured
Pubmed
Web of science
Create date
19/11/2007 12:22
Last modification date
20/08/2019 13:04
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