Pericentrin anchors protein kinase A at the centrosome through a newly identified RII-binding domain
Détails
ID Serval
serval:BIB_238ED6AF9EE2
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Pericentrin anchors protein kinase A at the centrosome through a newly identified RII-binding domain
Périodique
Current Biology
ISSN
0960-9822 (Print)
Statut éditorial
Publié
Date de publication
04/2000
Peer-reviewed
Oui
Volume
10
Numéro
7
Pages
417-20
Langue
anglais
Notes
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S. --- Old month value: Apr 6
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S. --- Old month value: Apr 6
Résumé
Centrosomes orchestrate microtubule nucleation and spindle assembly during cell division [1,2] and have long been recognized as major anchoring sites for cAMP-dependent protein kinase (PKA) [3,4]. Subcellular compartmentalization of PKA is achieved through the association of the PKA holoenzyme with A-kinase anchoring proteins (AKAPs) [5,6]. AKAPs have been shown to contain a conserved helical motif, responsible for binding to the type II regulatory subunit (RII) of PKA, and a specific targeting motif unique to each anchoring protein that directs the kinase to specific intracellular locations. Here, we show that pericentrin, an integral component of the pericentriolar matrix of the centrosome that has been shown to regulate centrosome assembly and organization, directly interacts with PKA through a newly identified binding domain. We demonstrate that both RII and the catalytic subunit of PKA coimmunoprecipitate with pericentrin isolated from HEK-293 cell extracts and that PKA catalytic activity is enriched in pericentrin immunoprecipitates. The interaction of pericentrin with RII is mediated through a binding domain of 100 amino acids which does not exhibit the structural characteristics of similar regions on conventional AKAPs. Collectively, these results provide strong evidence that pericentrin is an AKAP in vivo.
Mots-clé
Antigens/*metabolism Binding Sites Carrier Proteins/*metabolism Centrosome/*metabolism Cyclic AMP-Dependent Protein Kinases/*metabolism Microtubule-Associated Proteins/*metabolism Peptide Fragments/metabolism Protein Binding Protein Structure, Tertiary
Pubmed
Web of science
Open Access
Oui
Création de la notice
24/01/2008 13:14
Dernière modification de la notice
28/08/2024 15:02