Pericentrin anchors protein kinase A at the centrosome through a newly identified RII-binding domain
Details
Serval ID
serval:BIB_238ED6AF9EE2
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Pericentrin anchors protein kinase A at the centrosome through a newly identified RII-binding domain
Journal
Current Biology
ISSN
0960-9822 (Print)
Publication state
Published
Issued date
04/2000
Peer-reviewed
Oui
Volume
10
Number
7
Pages
417-20
Language
english
Notes
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S. --- Old month value: Apr 6
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S. --- Old month value: Apr 6
Abstract
Centrosomes orchestrate microtubule nucleation and spindle assembly during cell division [1,2] and have long been recognized as major anchoring sites for cAMP-dependent protein kinase (PKA) [3,4]. Subcellular compartmentalization of PKA is achieved through the association of the PKA holoenzyme with A-kinase anchoring proteins (AKAPs) [5,6]. AKAPs have been shown to contain a conserved helical motif, responsible for binding to the type II regulatory subunit (RII) of PKA, and a specific targeting motif unique to each anchoring protein that directs the kinase to specific intracellular locations. Here, we show that pericentrin, an integral component of the pericentriolar matrix of the centrosome that has been shown to regulate centrosome assembly and organization, directly interacts with PKA through a newly identified binding domain. We demonstrate that both RII and the catalytic subunit of PKA coimmunoprecipitate with pericentrin isolated from HEK-293 cell extracts and that PKA catalytic activity is enriched in pericentrin immunoprecipitates. The interaction of pericentrin with RII is mediated through a binding domain of 100 amino acids which does not exhibit the structural characteristics of similar regions on conventional AKAPs. Collectively, these results provide strong evidence that pericentrin is an AKAP in vivo.
Keywords
Antigens/*metabolism Binding Sites Carrier Proteins/*metabolism Centrosome/*metabolism Cyclic AMP-Dependent Protein Kinases/*metabolism Microtubule-Associated Proteins/*metabolism Peptide Fragments/metabolism Protein Binding Protein Structure, Tertiary
Pubmed
Web of science
Open Access
Yes
Create date
24/01/2008 13:14
Last modification date
28/08/2024 15:02