Spatial and temporal dynamics of jasmonate synthesis and accumulation in Arabidopsis in response to wounding.

Détails

ID Serval
serval:BIB_21A3C241877F
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Spatial and temporal dynamics of jasmonate synthesis and accumulation in Arabidopsis in response to wounding.
Périodique
Journal of Biological Chemistry
Auteur(s)
Glauser G., Grata E., Dubugnon L., Rudaz S., Farmer E.E., Wolfender J.L.
ISSN
0021-9258
Statut éditorial
Publié
Date de publication
2008
Peer-reviewed
Oui
Volume
283
Numéro
24
Pages
16400-16407
Langue
anglais
Notes
Publication types: Journal Article
Résumé
A new metabolite profiling approach combined with an ultrarapid sample preparation procedure was used to study the temporal and spatial dynamics of the wound-induced accumulation of jasmonic acid (JA) and its oxygenated derivatives in Arabidopsis thaliana. In addition to well known jasmonates, including hydroxyjasmonates (HOJAs), jasmonoyl-isoleucine (JA-Ile), and its 12-hydroxy derivative (12-HOJA-Ile), a new wound-induced dicarboxyjasmonate, 12-carboxyjasmonoyl-l-isoleucine (12-HOOCJA-Ile) was discovered. HOJAs and 12-HOOCJA-Ile were enriched in the midveins of wounded leaves, strongly differentiating them from the other jasmonate metabolites studied. The polarity of these oxylipins at physiological pH correlated with their appearance in midveins. When the time points of accumulation of different jasmonates were determined, JA levels were found to increase within 2-5 min of wounding. Remarkably, these changes occurred throughout the plant and were not restricted to wounded leaves. The speed of the stimulus leading to JA accumulation in leaves distal to a wound is at least 3 cm/min. The data give new insights into the spatial and temporal accumulation of jasmonates and have implications in the understanding of long-distance wound signaling in plants.
Mots-clé
Arabidopsis/metabolism, Arabidopsis Proteins/chemistry, Chromatography, Liquid/methods, Cyclopentanes/metabolism, Fatty Acids/metabolism, Gene Expression Regulation, Plant, Hydrogen-Ion Concentration, Magnetic Resonance Spectroscopy, Mass Spectrometry/methods, Models, Biological, Models, Chemical, Oxygen/metabolism, Oxylipins/metabolism, Plant Growth Regulators/metabolism, Plant Leaves/metabolism, Time Factors
Pubmed
Web of science
Open Access
Oui
Création de la notice
24/02/2009 11:46
Dernière modification de la notice
20/08/2019 12:58
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