Spatial and temporal dynamics of jasmonate synthesis and accumulation in Arabidopsis in response to wounding.

Details

Serval ID
serval:BIB_21A3C241877F
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Spatial and temporal dynamics of jasmonate synthesis and accumulation in Arabidopsis in response to wounding.
Journal
Journal of Biological Chemistry
Author(s)
Glauser G., Grata E., Dubugnon L., Rudaz S., Farmer E.E., Wolfender J.L.
ISSN
0021-9258
Publication state
Published
Issued date
2008
Peer-reviewed
Oui
Volume
283
Number
24
Pages
16400-16407
Language
english
Notes
Publication types: Journal Article
Abstract
A new metabolite profiling approach combined with an ultrarapid sample preparation procedure was used to study the temporal and spatial dynamics of the wound-induced accumulation of jasmonic acid (JA) and its oxygenated derivatives in Arabidopsis thaliana. In addition to well known jasmonates, including hydroxyjasmonates (HOJAs), jasmonoyl-isoleucine (JA-Ile), and its 12-hydroxy derivative (12-HOJA-Ile), a new wound-induced dicarboxyjasmonate, 12-carboxyjasmonoyl-l-isoleucine (12-HOOCJA-Ile) was discovered. HOJAs and 12-HOOCJA-Ile were enriched in the midveins of wounded leaves, strongly differentiating them from the other jasmonate metabolites studied. The polarity of these oxylipins at physiological pH correlated with their appearance in midveins. When the time points of accumulation of different jasmonates were determined, JA levels were found to increase within 2-5 min of wounding. Remarkably, these changes occurred throughout the plant and were not restricted to wounded leaves. The speed of the stimulus leading to JA accumulation in leaves distal to a wound is at least 3 cm/min. The data give new insights into the spatial and temporal accumulation of jasmonates and have implications in the understanding of long-distance wound signaling in plants.
Keywords
Arabidopsis/metabolism, Arabidopsis Proteins/chemistry, Chromatography, Liquid/methods, Cyclopentanes/metabolism, Fatty Acids/metabolism, Gene Expression Regulation, Plant, Hydrogen-Ion Concentration, Magnetic Resonance Spectroscopy, Mass Spectrometry/methods, Models, Biological, Models, Chemical, Oxygen/metabolism, Oxylipins/metabolism, Plant Growth Regulators/metabolism, Plant Leaves/metabolism, Time Factors
Pubmed
Web of science
Open Access
Yes
Create date
24/02/2009 11:46
Last modification date
20/08/2019 12:58
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