Genome of the carbapenemase-producing clinical isolate Elizabethkingia miricola EM_CHUV and comparative genomics with Elizabethkingia meningoseptica and Elizabethkingia anophelis: evidence for intrinsic multidrug resistance trait of emerging pathogens.

Détails

Ressource 1Télécharger: 5_27913093_Postprint.pdf (820.67 [Ko])
Etat: Public
Version: Author's accepted manuscript
ID Serval
serval:BIB_21829270BB0D
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Genome of the carbapenemase-producing clinical isolate Elizabethkingia miricola EM_CHUV and comparative genomics with Elizabethkingia meningoseptica and Elizabethkingia anophelis: evidence for intrinsic multidrug resistance trait of emerging pathogens.
Périodique
International journal of antimicrobial agents
Auteur(s)
Opota O., Diene S.M., Bertelli C., Prod'hom G., Eckert P., Greub G.
ISSN
1872-7913 (Electronic)
ISSN-L
0924-8579
Statut éditorial
Publié
Date de publication
01/2017
Peer-reviewed
Oui
Volume
49
Numéro
1
Pages
93-97
Langue
anglais
Notes
Publication types: Case Reports ; Comparative Study ; Journal Article
Publication Status: ppublish
Article; Bibliomics
Résumé
Elizabethkingia miricola is a Gram-negative non-fermenting rod emerging as a life-threatening human pathogen. The multidrug-resistant (MDR) carbapenemase-producing clinical isolate E. miricola EM_CHUV was recovered in the setting of severe nosocomial pneumonia. In this study, the genome of E. miricola EM_CHUV was sequenced and a functional analysis was performed, including a comparative genomic study with Elizabethkingia meningoseptica and Elizabethkingia anophelis. The resistome of EM_CHUV revealed the presence of a high number of resistance genes, including the presence of the blaGOB-13 and blaB-9 carbapenemase-encoding genes. Twelve mobility genes, with only two of them located in the proximity of resistance genes, and four potential genomic islands were identified in the genome of EM_CHUV, but no prophages or CRISPR sequences. Ten restriction-modification system (RMS) genes were also identified. In addition, we report the presence of a putative conjugative plasmid (pEM_CHUV) that does not encode any antibiotic resistance genes. Altogether, these findings point towards a limited number of DNA exchanges with other bacteria and suggest that multidrug resistance is an intrinsic trait of E. miricola owing to the presence of a high number of resistance genes within the bacterial core genome.

Mots-clé
Adult, Aged, 80 and over, Child, Preschool, Cross Infection/microbiology, Drug Resistance, Multiple, Bacterial, Female, Flavobacteriaceae/enzymology, Flavobacteriaceae/genetics, Flavobacteriaceae/isolation & purification, Flavobacteriaceae Infections/microbiology, Genes, Bacterial, Genome, Bacterial, Humans, Male, Middle Aged, Pneumonia, Bacterial/microbiology, Sequence Analysis, DNA
Pubmed
Web of science
Création de la notice
12/12/2016 19:51
Dernière modification de la notice
20/08/2019 12:58
Données d'usage