Social isolation stress and chronic glutathione deficiency have a common effect on the glutamine-to-glutamate ratio and myo-inositol concentration in the mouse frontal cortex.
Détails
Etat: Public
Version: Author's accepted manuscript
ID Serval
serval:BIB_1F8ECFA9EBB0
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Social isolation stress and chronic glutathione deficiency have a common effect on the glutamine-to-glutamate ratio and myo-inositol concentration in the mouse frontal cortex.
Périodique
Journal of neurochemistry
ISSN
1471-4159 (Electronic)
ISSN-L
0022-3042
Statut éditorial
Publié
Date de publication
09/2017
Peer-reviewed
Oui
Volume
142
Numéro
5
Pages
767-775
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: ppublish
Publication Status: ppublish
Résumé
Environmental stress can interact with genetic predisposition to increase the risk of developing psychopathology. In this work, we tested the hypothesis that social isolation stress interacts with impaired glutathione synthesis and have cumulative effects on the neurochemical profile of the frontal cortex. A mouse model with chronic glutathione deficit induced by knockout (-/-) of the glutamate-cysteine ligase modulatory subunit (Gclm) was exposed to social isolation stress from weaning to post-natal day 65. Using magnetic resonance methods at high-field (14.1 T), we analysed the neurochemical profile in the frontal cortex, brain size and ventricular volume of adult animals. Glutathione deficit was accompanied by elevated concentrations of N-acetylaspartate, alanine, and glutamine, as well as the ratio of glutamine-to-glutamate (Gln/Glu), and by a reduction in levels of myo-inositol and choline-containing compounds in the frontal cortex of -/- animals with respect to wild-type littermates. Although there was no significant interaction between social isolation stress and glutathione deficiency, mice reared in isolation displayed lower myo-inositol concentration (-8.4%, p < 0.05) and larger Gln/Glu (+7.6%, p < 0.05), relative to those in group housing. Furthermore, glutathione deficiency caused a reduction in whole brain volume and enlargement of ventricles, but social isolation had no effect on these parameters. We conclude that social isolation caused neurochemical alterations that may add to those associated to impaired glutathione synthesis.
Mots-clé
Animals, Frontal Lobe/metabolism, Glutamic Acid/metabolism, Glutamine/metabolism, Glutathione/deficiency, Inositol/metabolism, Male, Mice, Mice, Inbred C57BL, Mice, Knockout, Social Isolation/psychology, Stress, Psychological/metabolism, Stress, Psychological/psychology, magnetic resonance spectroscopy, metabolism, neurochemical profile, neurodevelopmental, social isolation
Pubmed
Web of science
Open Access
Oui
Création de la notice
06/07/2017 9:47
Dernière modification de la notice
20/08/2019 13:55