The nuclear matrix: a critical appraisal.

Détails

ID Serval
serval:BIB_1F4E79610D01
Type
Article: article d'un périodique ou d'un magazine.
Sous-type
Synthèse (review): revue aussi complète que possible des connaissances sur un sujet, rédigée à partir de l'analyse exhaustive des travaux publiés.
Collection
Publications
Institution
Titre
The nuclear matrix: a critical appraisal.
Périodique
Histology and Histopathology
Auteur(s)
Martelli A.M., Cocco L., Riederer B.M., Neri L.M.
ISSN
0213-3911 (Print)
ISSN-L
0213-3911
Statut éditorial
Publié
Date de publication
1996
Volume
11
Numéro
4
Pages
1035-1048
Langue
anglais
Résumé
It is becoming increasingly clear that the cell nucleus is a highly structurized organelle. Because of its tight compartmentalization, it is generally believed that a framework must exist, responsible for maintaining such a spatial organization. Over the last twenty years many investigations have been devoted to identifying the nuclear framework. Structures isolated by different techniques have been obtained in vitro and are variously referred to as nuclear matrix, nucleoskeleton or nuclear scaffold. Many different functions, such as DNA replication and repair, mRNA transcription, processing and transport have been described to occur in close association with these structures. However, there is still much debate as to whether or not any of these preparations corresponds to a nuclear framework that exists in vivo. In this article we summarize the most commonly-used methods for obtaining preparations of nuclear frameworks and we also stress the possible artifacts that can be created in vitro during the isolation procedures. Emphasis is placed also on the protein composition of the frameworks as well as on some possible signalling functions that have been recently described to occur in tight association with the nuclear matrix.
Mots-clé
Animals, Cell Nucleus/physiology, Cell Nucleus/ultrastructure, Chromatin/ultrastructure, DNA Repair, DNA Replication, Humans, Nuclear Matrix/physiology, Nuclear Matrix/ultrastructure, RNA, Messenger/biosynthesis, Staining and Labeling, Transcription, Genetic
Pubmed
Web of science
Création de la notice
24/01/2008 14:34
Dernière modification de la notice
20/08/2019 12:55
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