The nuclear matrix: a critical appraisal.

Details

Serval ID
serval:BIB_1F4E79610D01
Type
Article: article from journal or magazin.
Publication sub-type
Review (review): journal as complete as possible of one specific subject, written based on exhaustive analyses from published work.
Collection
Publications
Institution
Title
The nuclear matrix: a critical appraisal.
Journal
Histology and Histopathology
Author(s)
Martelli A.M., Cocco L., Riederer B.M., Neri L.M.
ISSN
0213-3911 (Print)
ISSN-L
0213-3911
Publication state
Published
Issued date
1996
Volume
11
Number
4
Pages
1035-1048
Language
english
Abstract
It is becoming increasingly clear that the cell nucleus is a highly structurized organelle. Because of its tight compartmentalization, it is generally believed that a framework must exist, responsible for maintaining such a spatial organization. Over the last twenty years many investigations have been devoted to identifying the nuclear framework. Structures isolated by different techniques have been obtained in vitro and are variously referred to as nuclear matrix, nucleoskeleton or nuclear scaffold. Many different functions, such as DNA replication and repair, mRNA transcription, processing and transport have been described to occur in close association with these structures. However, there is still much debate as to whether or not any of these preparations corresponds to a nuclear framework that exists in vivo. In this article we summarize the most commonly-used methods for obtaining preparations of nuclear frameworks and we also stress the possible artifacts that can be created in vitro during the isolation procedures. Emphasis is placed also on the protein composition of the frameworks as well as on some possible signalling functions that have been recently described to occur in tight association with the nuclear matrix.
Keywords
Animals, Cell Nucleus/physiology, Cell Nucleus/ultrastructure, Chromatin/ultrastructure, DNA Repair, DNA Replication, Humans, Nuclear Matrix/physiology, Nuclear Matrix/ultrastructure, RNA, Messenger/biosynthesis, Staining and Labeling, Transcription, Genetic
Pubmed
Web of science
Create date
24/01/2008 15:34
Last modification date
20/08/2019 13:55
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