High-Level Resistance to Colistin Mediated by Various Mutations in the crrB Gene among Carbapenemase-Producing Klebsiella pneumoniae.

Jayol, A.; Nordmann, P.; Brink, A.; Villegas, M.V.; Dubois, V.; Poirel, L.

doi:10.1128/AAC.01423-17

High-Level Resistance to Colistin Mediated by Various Mutations in the crrB Gene among Carbapenemase-Producing Klebsiella pneumoniae.

Détails

Demande d'une copie

ID Serval

serval:BIB_1B792292EF1C

Type

Article: article d'un périodique ou d'un magazine.

Collection

Publications

Institution

UNIL/CHUV

Titre

High-Level Resistance to Colistin Mediated by Various Mutations in the crrB Gene among Carbapenemase-Producing Klebsiella pneumoniae.

Périodique

Antimicrobial agents and chemotherapy

Auteur⸱e⸱s

Jayol A., Nordmann P., Brink A., Villegas M.V., Dubois V., Poirel L.

ISSN

1098-6596 (Electronic)

ISSN-L

0066-4804

Statut éditorial

Publié

Date de publication

11/2017

Peer-reviewed

Oui

Volume

Numéro

Pages

1-4

Langue

anglais

Notes

Publication types: Journal Article
Publication Status: epublish

Résumé

Mutations in crrAB genes encoding a two-component regulator involved in modifications of lipopolysaccharide were searched for among a collection of colistin-resistant Klebsiella pneumoniae isolates. Four isolates, respectively, producing carbapenemases NDM-1, OXA-181, or KPC-2 showed mutated CrrB proteins compared with those in wild-type strains. Complementation assays with a wild-type CrrB protein restored the susceptibility to colistin in all cases, confirming the involvement of the identified substitutions in the resistance phenotype.

Mots-clé

Amino Acid Substitution, Anti-Bacterial Agents/pharmacology, Bacterial Proteins/genetics, Bacterial Proteins/metabolism, Colistin/pharmacology, Drug Resistance, Bacterial/drug effects, Drug Resistance, Bacterial/genetics, Gene Expression Regulation, Bacterial/drug effects, Genetic Complementation Test, Humans, Klebsiella pneumoniae/drug effects, Klebsiella pneumoniae/genetics, Klebsiella pneumoniae/isolation & purification, Klebsiella pneumoniae/metabolism, Microbial Sensitivity Tests, Mutation, beta-Lactamases/genetics, beta-Lactamases/metabolism, CrrAB, Klebsiella pneumoniae, polymyxin, rapid polymyxin NP test, resistance mechanisms, susceptibility testing

OAI-PMH

oai:serval.unil.ch:BIB_1B792292EF1C

DOI

10.1128/AAC.01423-17

Pubmed

28874377

Web of science

000413558300020

Open Access

Oui

Création de la notice

07/09/2017 16:14