High-Level Resistance to Colistin Mediated by Various Mutations in the <i>crrB</i> Gene among Carbapenemase-Producing Klebsiella pneumoniae.

Détails

ID Serval
serval:BIB_1B792292EF1C
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
High-Level Resistance to Colistin Mediated by Various Mutations in the <i>crrB</i> Gene among Carbapenemase-Producing Klebsiella pneumoniae.
Périodique
Antimicrobial agents and chemotherapy
Auteur⸱e⸱s
Jayol A., Nordmann P., Brink A., Villegas M.V., Dubois V., Poirel L.
ISSN
1098-6596 (Electronic)
ISSN-L
0066-4804
Statut éditorial
Publié
Date de publication
11/2017
Peer-reviewed
Oui
Volume
61
Numéro
11
Pages
1-4
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: epublish
Résumé
Mutations in <i>crrAB</i> genes encoding a two-component regulator involved in modifications of lipopolysaccharide were searched for among a collection of colistin-resistant <i>Klebsiella pneumoniae</i> isolates. Four isolates, respectively, producing carbapenemases NDM-1, OXA-181, or KPC-2 showed mutated CrrB proteins compared with those in wild-type strains. Complementation assays with a wild-type CrrB protein restored the susceptibility to colistin in all cases, confirming the involvement of the identified substitutions in the resistance phenotype.
Mots-clé
Amino Acid Substitution, Anti-Bacterial Agents/pharmacology, Bacterial Proteins/genetics, Bacterial Proteins/metabolism, Colistin/pharmacology, Drug Resistance, Bacterial/drug effects, Drug Resistance, Bacterial/genetics, Gene Expression Regulation, Bacterial/drug effects, Genetic Complementation Test, Humans, Klebsiella pneumoniae/drug effects, Klebsiella pneumoniae/genetics, Klebsiella pneumoniae/isolation & purification, Klebsiella pneumoniae/metabolism, Microbial Sensitivity Tests, Mutation, beta-Lactamases/genetics, beta-Lactamases/metabolism, CrrAB, Klebsiella pneumoniae, polymyxin, rapid polymyxin NP test, resistance mechanisms, susceptibility testing
Pubmed
Web of science
Open Access
Oui
Création de la notice
07/09/2017 15:14
Dernière modification de la notice
20/08/2019 12:52
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