High-Level Resistance to Colistin Mediated by Various Mutations in the crrB Gene among Carbapenemase-Producing Klebsiella pneumoniae.

Jayol, A.; Nordmann, P.; Brink, A.; Villegas, M.V.; Dubois, V.; Poirel, L.

doi:10.1128/AAC.01423-17

High-Level Resistance to Colistin Mediated by Various Mutations in the crrB Gene among Carbapenemase-Producing Klebsiella pneumoniae.

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Serval ID

serval:BIB_1B792292EF1C

Type

Article: article from journal or magazin.

Collection

Publications

Institution

UNIL/CHUV

Title

High-Level Resistance to Colistin Mediated by Various Mutations in the crrB Gene among Carbapenemase-Producing Klebsiella pneumoniae.

Journal

Antimicrobial agents and chemotherapy

Author(s)

Jayol A., Nordmann P., Brink A., Villegas M.V., Dubois V., Poirel L.

ISSN

1098-6596 (Electronic)

ISSN-L

0066-4804

Publication state

Published

Issued date

11/2017

Peer-reviewed

Oui

Volume

Number

Pages

1-4

Language

english

Notes

Publication types: Journal Article
Publication Status: epublish

Abstract

Mutations in crrAB genes encoding a two-component regulator involved in modifications of lipopolysaccharide were searched for among a collection of colistin-resistant Klebsiella pneumoniae isolates. Four isolates, respectively, producing carbapenemases NDM-1, OXA-181, or KPC-2 showed mutated CrrB proteins compared with those in wild-type strains. Complementation assays with a wild-type CrrB protein restored the susceptibility to colistin in all cases, confirming the involvement of the identified substitutions in the resistance phenotype.

Keywords

Amino Acid Substitution, Anti-Bacterial Agents/pharmacology, Bacterial Proteins/genetics, Bacterial Proteins/metabolism, Colistin/pharmacology, Drug Resistance, Bacterial/drug effects, Drug Resistance, Bacterial/genetics, Gene Expression Regulation, Bacterial/drug effects, Genetic Complementation Test, Humans, Klebsiella pneumoniae/drug effects, Klebsiella pneumoniae/genetics, Klebsiella pneumoniae/isolation & purification, Klebsiella pneumoniae/metabolism, Microbial Sensitivity Tests, Mutation, beta-Lactamases/genetics, beta-Lactamases/metabolism, CrrAB, Klebsiella pneumoniae, polymyxin, rapid polymyxin NP test, resistance mechanisms, susceptibility testing

OAI-PMH

oai:serval.unil.ch:BIB_1B792292EF1C

DOI

10.1128/AAC.01423-17

Pubmed

28874377

Web of science

000413558300020

Open Access

Yes

Create date

07/09/2017 16:14