FtsK Is a DNA motor protein that activates chromosome dimer resolution by switching the catalytic state of the XerC and XerD recombinases.

Détails

ID Serval
serval:BIB_194ACF6725B5
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
FtsK Is a DNA motor protein that activates chromosome dimer resolution by switching the catalytic state of the XerC and XerD recombinases.
Périodique
Cell
Auteur(s)
Aussel L., Barre F.X., Aroyo M., Stasiak A., Stasiak A.Z., Sherratt D.
ISSN
0092-8674[print], 0092-8674[linking]
Statut éditorial
Publié
Date de publication
01/2002
Volume
108
Numéro
2
Pages
195-205
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
FtsK acts at the bacterial division septum to couple chromosome segregation with cell division. We demonstrate that a truncated FtsK derivative, FtsK(50C), uses ATP hydrolysis to translocate along duplex DNA as a multimer in vitro, consistent with FtsK having an in vivo role in pumping DNA through the closing division septum. FtsK(50C) also promotes a complete Xer recombination reaction between dif sites by switching the state of activity of the XerCD recombinases so that XerD makes the first pair of strand exchanges to form Holliday junctions that are then resolved by XerC. The reaction between directly repeated dif sites in circular DNA leads to the formation of uncatenated circles and is equivalent to the formation of chromosome monomers from dimers.
Mots-clé
Adenosine Triphosphatases/metabolism, Adenosine Triphosphate/metabolism, Bacterial Proteins/chemistry, Bacterial Proteins/metabolism, Catalysis, DNA Nucleotidyltransferases/chemistry, DNA Nucleotidyltransferases/metabolism, DNA, Bacterial/chemistry, DNA, Bacterial/metabolism, DNA, Superhelical/chemistry, DNA, Superhelical/metabolism, Dimerization, Escherichia coli Proteins, Hydrolysis, Integrases, Membrane Proteins/chemistry, Membrane Proteins/metabolism, Models, Biological, Molecular Motor Proteins/chemistry, Molecular Motor Proteins/metabolism, Nucleic Acid Conformation, Protein Conformation, Recombinases, Recombination, Genetic/physiology
Pubmed
Web of science
Open Access
Oui
Création de la notice
24/01/2008 11:36
Dernière modification de la notice
20/08/2019 13:50
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