FtsK Is a DNA motor protein that activates chromosome dimer resolution by switching the catalytic state of the XerC and XerD recombinases.

Details

Serval ID
serval:BIB_194ACF6725B5
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
FtsK Is a DNA motor protein that activates chromosome dimer resolution by switching the catalytic state of the XerC and XerD recombinases.
Journal
Cell
Author(s)
Aussel L., Barre F.X., Aroyo M., Stasiak A., Stasiak A.Z., Sherratt D.
ISSN
0092-8674[print], 0092-8674[linking]
Publication state
Published
Issued date
01/2002
Volume
108
Number
2
Pages
195-205
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
FtsK acts at the bacterial division septum to couple chromosome segregation with cell division. We demonstrate that a truncated FtsK derivative, FtsK(50C), uses ATP hydrolysis to translocate along duplex DNA as a multimer in vitro, consistent with FtsK having an in vivo role in pumping DNA through the closing division septum. FtsK(50C) also promotes a complete Xer recombination reaction between dif sites by switching the state of activity of the XerCD recombinases so that XerD makes the first pair of strand exchanges to form Holliday junctions that are then resolved by XerC. The reaction between directly repeated dif sites in circular DNA leads to the formation of uncatenated circles and is equivalent to the formation of chromosome monomers from dimers.
Keywords
Adenosine Triphosphatases/metabolism, Adenosine Triphosphate/metabolism, Bacterial Proteins/chemistry, Bacterial Proteins/metabolism, Catalysis, DNA Nucleotidyltransferases/chemistry, DNA Nucleotidyltransferases/metabolism, DNA, Bacterial/chemistry, DNA, Bacterial/metabolism, DNA, Superhelical/chemistry, DNA, Superhelical/metabolism, Dimerization, Escherichia coli Proteins, Hydrolysis, Integrases, Membrane Proteins/chemistry, Membrane Proteins/metabolism, Models, Biological, Molecular Motor Proteins/chemistry, Molecular Motor Proteins/metabolism, Nucleic Acid Conformation, Protein Conformation, Recombinases, Recombination, Genetic/physiology
Pubmed
Web of science
Open Access
Yes
Create date
24/01/2008 10:36
Last modification date
20/08/2019 12:50
Usage data