Interactions of interferon-alpha 2a with 5'-deoxy-5-fluorouridine in colorectal cancer cells in vitro

Détails

ID Serval
serval:BIB_178ADBE529F5
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Interactions of interferon-alpha 2a with 5'-deoxy-5-fluorouridine in colorectal cancer cells in vitro
Périodique
European Journal of Cancer
Auteur(s)
Tevaearai  H. T., Laurent  P. L., Suardet  L., Eliason  J. F., Givel  J. C., Odartchenko  N.
ISSN
0959-8049 (Print)
Statut éditorial
Publié
Date de publication
1992
Volume
28
Numéro
2-3
Pages
368-72
Notes
Journal Article
Research Support, Non-U.S. Gov't
Résumé
The biological activity of 5'-deoxy-5-fluorouridine (5'-dFUrd) depends upon intracellular enzymatic cleavage by pyrimidine phosphorylase to form 5-fluorouracil (5-FU). Interferon-alpha 2a (IFN-alpha) effect was analysed alone and combined with 5-FU or 5'-dFUrd, on proliferation inhibition of eight human colorectal cancer cell lines. The toxicity of 5-FU was enhanced by IFN-alpha in only one line (SW-480). In contrast, interactive enhancement of IFN-alpha was observed with 5'-dFUrd in five lines (WiDr, HT-29, 513, SW-480 and Co-115). In each of the lines showing potentiation by IFN/5'dFUrd but not by IFN/5-FU, cytoplasmic pyrimidine phosphorylase activity was increased after 5 days' incubation with IFN-alpha in a dose-dependent manner. Two lines (LISP-1 and SW-620) showed no potentiation of either 5-FU or 5'-dFUrd toxicity by IFN-alpha, and no change in pyrimidine phosphorylase activity. Potentiation of 5'-dFUrd effect by IFN-alpha may thus be explained by an enhancement of its conversion to 5-FU through stimulation of pyrimidine phosphorylase activity.
Mots-clé
Antineoplastic Agents/*pharmacology Cell Division/drug effects Colorectal Neoplasms/enzymology/*pathology Dose-Response Relationship, Drug Floxuridine/*pharmacology Humans Interferon Alfa-2a/*pharmacology Thymidine Phosphorylase/drug effects Tumor Cells, Cultured/drug effects
Pubmed
Web of science
Création de la notice
28/01/2008 8:56
Dernière modification de la notice
20/08/2019 12:47
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