Interactions of interferon-alpha 2a with 5'-deoxy-5-fluorouridine in colorectal cancer cells in vitro
Details
Serval ID
serval:BIB_178ADBE529F5
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Interactions of interferon-alpha 2a with 5'-deoxy-5-fluorouridine in colorectal cancer cells in vitro
Journal
European Journal of Cancer
ISSN
0959-8049 (Print)
Publication state
Published
Issued date
1992
Volume
28
Number
2-3
Pages
368-72
Notes
Journal Article
Research Support, Non-U.S. Gov't
Research Support, Non-U.S. Gov't
Abstract
The biological activity of 5'-deoxy-5-fluorouridine (5'-dFUrd) depends upon intracellular enzymatic cleavage by pyrimidine phosphorylase to form 5-fluorouracil (5-FU). Interferon-alpha 2a (IFN-alpha) effect was analysed alone and combined with 5-FU or 5'-dFUrd, on proliferation inhibition of eight human colorectal cancer cell lines. The toxicity of 5-FU was enhanced by IFN-alpha in only one line (SW-480). In contrast, interactive enhancement of IFN-alpha was observed with 5'-dFUrd in five lines (WiDr, HT-29, 513, SW-480 and Co-115). In each of the lines showing potentiation by IFN/5'dFUrd but not by IFN/5-FU, cytoplasmic pyrimidine phosphorylase activity was increased after 5 days' incubation with IFN-alpha in a dose-dependent manner. Two lines (LISP-1 and SW-620) showed no potentiation of either 5-FU or 5'-dFUrd toxicity by IFN-alpha, and no change in pyrimidine phosphorylase activity. Potentiation of 5'-dFUrd effect by IFN-alpha may thus be explained by an enhancement of its conversion to 5-FU through stimulation of pyrimidine phosphorylase activity.
Keywords
Antineoplastic Agents/*pharmacology
Cell Division/drug effects
Colorectal Neoplasms/enzymology/*pathology
Dose-Response Relationship, Drug
Floxuridine/*pharmacology
Humans
Interferon Alfa-2a/*pharmacology
Thymidine Phosphorylase/drug effects
Tumor Cells, Cultured/drug effects
Pubmed
Web of science
Create date
28/01/2008 9:56
Last modification date
20/08/2019 13:47