Interactions of interferon-alpha 2a with 5'-deoxy-5-fluorouridine in colorectal cancer cells in vitro

Details

Serval ID
serval:BIB_178ADBE529F5
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Interactions of interferon-alpha 2a with 5'-deoxy-5-fluorouridine in colorectal cancer cells in vitro
Journal
European Journal of Cancer
Author(s)
Tevaearai  H. T., Laurent  P. L., Suardet  L., Eliason  J. F., Givel  J. C., Odartchenko  N.
ISSN
0959-8049 (Print)
Publication state
Published
Issued date
1992
Volume
28
Number
2-3
Pages
368-72
Notes
Journal Article
Research Support, Non-U.S. Gov't
Abstract
The biological activity of 5'-deoxy-5-fluorouridine (5'-dFUrd) depends upon intracellular enzymatic cleavage by pyrimidine phosphorylase to form 5-fluorouracil (5-FU). Interferon-alpha 2a (IFN-alpha) effect was analysed alone and combined with 5-FU or 5'-dFUrd, on proliferation inhibition of eight human colorectal cancer cell lines. The toxicity of 5-FU was enhanced by IFN-alpha in only one line (SW-480). In contrast, interactive enhancement of IFN-alpha was observed with 5'-dFUrd in five lines (WiDr, HT-29, 513, SW-480 and Co-115). In each of the lines showing potentiation by IFN/5'dFUrd but not by IFN/5-FU, cytoplasmic pyrimidine phosphorylase activity was increased after 5 days' incubation with IFN-alpha in a dose-dependent manner. Two lines (LISP-1 and SW-620) showed no potentiation of either 5-FU or 5'-dFUrd toxicity by IFN-alpha, and no change in pyrimidine phosphorylase activity. Potentiation of 5'-dFUrd effect by IFN-alpha may thus be explained by an enhancement of its conversion to 5-FU through stimulation of pyrimidine phosphorylase activity.
Keywords
Antineoplastic Agents/*pharmacology Cell Division/drug effects Colorectal Neoplasms/enzymology/*pathology Dose-Response Relationship, Drug Floxuridine/*pharmacology Humans Interferon Alfa-2a/*pharmacology Thymidine Phosphorylase/drug effects Tumor Cells, Cultured/drug effects
Pubmed
Web of science
Create date
28/01/2008 8:56
Last modification date
20/08/2019 12:47
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