Microbial diagnosis of bloodstream infection: towards molecular diagnosis directly from blood.
Détails
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Accès restreint UNIL
Etat: Public
Version: Final published version
ID Serval
serval:BIB_107D523BF53E
Type
Article: article d'un périodique ou d'un magazine.
Sous-type
Synthèse (review): revue aussi complète que possible des connaissances sur un sujet, rédigée à partir de l'analyse exhaustive des travaux publiés.
Collection
Publications
Institution
Titre
Microbial diagnosis of bloodstream infection: towards molecular diagnosis directly from blood.
Périodique
Clinical Microbiology and Infection
ISSN
1469-0691 (Electronic)
ISSN-L
1198-743X
Statut éditorial
Publié
Date de publication
2015
Peer-reviewed
Oui
Volume
21
Numéro
4
Pages
323-331
Langue
anglais
Notes
Publication types: <Publication Status: ppublish Document Type: Review
Résumé
When a bloodstream infection (BSI) is suspected, most of the laboratory results-biochemical and haematologic-are available within the first hours after hospital admission of the patient. This is not the case for diagnostic microbiology, which generally takes a longer time because blood culture, which is to date the reference standard for the documentation of the BSI microbial agents, relies on bacterial or fungal growth. The microbial diagnosis of BSI directly from blood has been proposed to speed the determination of the etiological agent but was limited by the very low number of circulating microbes during these paucibacterial infections. Thanks to recent advances in molecular biology, including the improvement of nucleic acid extraction and amplification, several PCR-based methods for the diagnosis of BSI directly from whole blood have emerged. In the present review, we discuss the advantages and limitations of these new molecular approaches, which at best complement the culture-based diagnosis of BSI.
Pubmed
Web of science
Open Access
Oui
Création de la notice
12/06/2015 17:47
Dernière modification de la notice
20/08/2019 13:37