Microbial diagnosis of bloodstream infection: towards molecular diagnosis directly from blood.

Details

Ressource 1Request a copy Sous embargo indéterminé.
State: Public
Version: Final published version
Serval ID
serval:BIB_107D523BF53E
Type
Article: article from journal or magazin.
Publication sub-type
Review (review): journal as complete as possible of one specific subject, written based on exhaustive analyses from published work.
Collection
Publications
Institution
Title
Microbial diagnosis of bloodstream infection: towards molecular diagnosis directly from blood.
Journal
Clinical Microbiology and Infection
Author(s)
Opota O., Jaton K., Greub G.
ISSN
1469-0691 (Electronic)
ISSN-L
1198-743X
Publication state
Published
Issued date
2015
Peer-reviewed
Oui
Volume
21
Number
4
Pages
323-331
Language
english
Notes
Publication types: <Publication Status: ppublish Document Type: Review
Abstract
When a bloodstream infection (BSI) is suspected, most of the laboratory results-biochemical and haematologic-are available within the first hours after hospital admission of the patient. This is not the case for diagnostic microbiology, which generally takes a longer time because blood culture, which is to date the reference standard for the documentation of the BSI microbial agents, relies on bacterial or fungal growth. The microbial diagnosis of BSI directly from blood has been proposed to speed the determination of the etiological agent but was limited by the very low number of circulating microbes during these paucibacterial infections. Thanks to recent advances in molecular biology, including the improvement of nucleic acid extraction and amplification, several PCR-based methods for the diagnosis of BSI directly from whole blood have emerged. In the present review, we discuss the advantages and limitations of these new molecular approaches, which at best complement the culture-based diagnosis of BSI.
Pubmed
Web of science
Open Access
Yes
Create date
12/06/2015 17:47
Last modification date
20/08/2019 13:37
Usage data