Proteomic analysis of cytokine induced proteins in human intestinal epithelial cells: implications for inflammatory bowel diseases.

Détails

ID Serval
serval:BIB_0CCACF3928B4
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Proteomic analysis of cytokine induced proteins in human intestinal epithelial cells: implications for inflammatory bowel diseases.
Périodique
Proteomics
Auteur⸱e⸱s
Barceló-Batllori S., André M., Servis C., Lévy N., Takikawa O., Michetti P., Reymond M., Felley-Bosco E.
ISSN
1615-9853
Statut éditorial
Publié
Date de publication
2002
Peer-reviewed
Oui
Volume
2
Numéro
5
Pages
551-60
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't - Publication Status: ppublish
Résumé
A role for cytokine regulated proteins in epithelial cells has been suggested in the pathogenesis of inflammatory bowel diseases (IBD). The aim of this study was to identify such cytokine regulated targets using a proteomic functional approach. Protein patterns from (35)S-radiolabeled homogenates of cultured colon epithelial cells were compared before and after exposure to interferon-gamma, interleukin-1beta and interleukin-6. Proteins were separated by two-dimensional polyacrylamide gel electrophoresis. Both autoradiographies and silver stained gels were analyzed. Proteins showing differential expression were identified by tryptic in-gel digestion and mass spectrometry. Metabolism related proteins were also investigated by Western blot analysis. Tryptophanyl-tRNA synthetase, indoleamine-2,3-dioxygenase, heterogeneous nuclear ribonucleoprotein JKTBP, interferon-induced 35kDa protein, proteasome subunit LMP2 and arginosuccinate synthetase were identified as cytokine modulated proteins in vitro. Using purified epithelial cells from patients, overexpression of indoleamine-2,3-dioxygenase, an enzyme involved in tryptophan metabolism, was confirmed in Crohn's disease as well as in ulcerative colitis, as compared to normal mucosa. No such difference was found in diverticulitis. Potentially, this observation opens new avenues in the treatment of IBD.
Mots-clé
Argininosuccinate Synthase, Cells, Cultured, Cytokines, Electrophoresis, Gel, Two-Dimensional, Epithelial Cells, Humans, Inflammatory Bowel Diseases, Intestinal Mucosa, Models, Biological, Proteome, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Tryptophan Oxygenase, Tryptophan-tRNA Ligase
Pubmed
Web of science
Création de la notice
25/01/2008 16:02
Dernière modification de la notice
20/08/2019 12:34
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